Live Cell/TIRF AF6000, BIOTEC
From BioDIP
(Difference between revisions)
| Line 7: | Line 7: | ||
|building=Biotec | |building=Biotec | ||
|room=226 | |room=226 | ||
| − | | | + | |Facility-logo=Imaging@Biotec.jpg |
| − | | | + | |Institute-logo=LOGO CRTD.jpg |
|description=Our Leica AFLX6000 TIRF is designed for observation of LiveCell systems (incubator) and phenomenons at the interface between the cells and their substrate. The laser lines are well distributed over the spectrum and especially good for common dye combinations. A system of external emission filters wheel allows extremely fast acquisition in combination with an EMCCD for detection of low signal. | |description=Our Leica AFLX6000 TIRF is designed for observation of LiveCell systems (incubator) and phenomenons at the interface between the cells and their substrate. The laser lines are well distributed over the spectrum and especially good for common dye combinations. A system of external emission filters wheel allows extremely fast acquisition in combination with an EMCCD for detection of low signal. | ||
|applications=- | |applications=- | ||
|image=Bioz06 - Leica AFLX6000 TIRF.jpg | |image=Bioz06 - Leica AFLX6000 TIRF.jpg | ||
| + | |stand-name=Leica - DMRE | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=motorized XY stage, Super Z-stage, fluorescence, transmitted light with automatic DIC, TIRF | |microscope=motorized XY stage, Super Z-stage, fluorescence, transmitted light with automatic DIC, TIRF | ||
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|reflectors=empty<br>FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30<br>CY5 : EX 635/10 ; BS 650 ; EM BP 720/60<br>Quad Laser Line<br>VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65<br>RFP : EX 552/24 ; BS 570 ; EX 605/65 | |reflectors=empty<br>FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30<br>CY5 : EX 635/10 ; BS 650 ; EM BP 720/60<br>Quad Laser Line<br>VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65<br>RFP : EX 552/24 ; BS 570 ; EX 605/65 | ||
|features=sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD) | |features=sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD) | ||
| + | |software=- | ||
|incubation=Cage incubator (T) | |incubation=Cage incubator (T) | ||
|link1=- | |link1=- | ||
|link2=- | |link2=- | ||
|inv=0 | |inv=0 | ||
| + | |facility_image=Imaging@Biotec.jpg | ||
| + | |logo_image=LOGO_CRTD.jpg | ||
|objectives=- | |objectives=- | ||
|illumination=laser (nm): 405, 488, 561, 635 | |illumination=laser (nm): 405, 488, 561, 635 | ||
}} | }} | ||
Revision as of 17:30, 26 November 2010
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Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | Leica - DMRE, Inverted stand, motorized XY stage, Super Z-stage, fluorescence, transmitted light with automatic DIC, TIRF | ||
| objectives | |||
| illumination | |||
| detection | Andor EMCCD (512*512, 16µm pixels), Leica DFC (1392*1040, 6,45µm pixels), reflectors for fluorescence and TIRF, external emission filter wheel for rapid acquisition with Andor EMCCD | ||
| reflectors | empty FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30 CY5 : EX 635/10 ; BS 650 ; EM BP 720/60 Quad Laser Line VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65 RFP : EX 552/24 ; BS 570 ; EX 605/65 | ||
| features | sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD) | ||
| software | - | ||
| incubation | Cage incubator (T) | ||
| links |
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| inv.nr. | 0 |
