Live Cell/TIRF AF6000, BIOTEC

From BioDIP
(Difference between revisions)
Jump to: navigation, search
Line 21: Line 21:
 
|obj7=-
 
|obj7=-
 
|obj8=-
 
|obj8=-
|ill1=-
+
|ill1=Laser Diode 405 nm
|ill2=-
+
|ill2=Laser DPSS 488 nm
|ill3=-
+
|ill3=Laser DPSS 561 nm
|ill4=-
+
|ill4=Laser HeNe 633 nm
 
|ill5=-
 
|ill5=-
 
|ill6=-
 
|ill6=-
Line 31: Line 31:
 
|detection=Andor EMCCD (512*512, 16µm pixels), Leica DFC (1392*1040, 6,45µm pixels), reflectors for fluorescence and TIRF, external emission filter wheel for rapid acquisition with Andor EMCCD
 
|detection=Andor EMCCD (512*512, 16µm pixels), Leica DFC (1392*1040, 6,45µm pixels), reflectors for fluorescence and TIRF, external emission filter wheel for rapid acquisition with Andor EMCCD
 
|reflectors=empty<br>FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30<br>CY5 : EX 635/10 ; BS 650 ; EM BP 720/60<br>Quad Laser Line<br>VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65<br>RFP : EX 552/24 ; BS 570 ; EX 605/65
 
|reflectors=empty<br>FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30<br>CY5 : EX 635/10 ; BS 650 ; EM BP 720/60<br>Quad Laser Line<br>VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65<br>RFP : EX 552/24 ; BS 570 ; EX 605/65
 
 
|features=sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD)
 
|features=sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD)
 
|incubation=cage incubator (T)
 
|incubation=cage incubator (T)

Revision as of 14:25, 7 September 2009

Suitable for -.
No image.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] Imaging@Biotec"Imaging@Biotec" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. ("Biotec" is not in the list of possible values (BIOTEC, CRTD, DZNE, MTZ, MPI-CBG) for this property., 226)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope , Inverted stand, motorized XY stage, Super Z-stage, fluorescence, transmitted light with automatic DIC, TIRF
objectives
Leica HC PL APO 10x 0.4
Leica HC PL APO 20x 0.7
-
-
-
-
-
-
illumination
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
-
-
-
-
detection Andor EMCCD (512*512, 16µm pixels), Leica DFC (1392*1040, 6,45µm pixels), reflectors for fluorescence and TIRF, external emission filter wheel for rapid acquisition with Andor EMCCD
reflectors empty
FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30
CY5 : EX 635/10 ; BS 650 ; EM BP 720/60
Quad Laser Line
VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65
RFP : EX 552/24 ; BS 570 ; EX 605/65
features sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD)
software
incubation cage incubator (T)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 0



Personal tools