Live Cell 4D, BIOTEC
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=BIO06 |
| − | |system-name=4D | + | |system-name=Live Cell 4D, BIOTEC |
|type=Microscope | |type=Microscope | ||
|category=Wide-Field | |category=Wide-Field | ||
| − | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
| − | |building= | + | |building=BIOTEC |
|room=226 | |room=226 | ||
|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
| − | |Institute-logo= | + | |Institute-logo=Biotec logo.png |
|description=Our Zeiss Axiovert 200 4D-System is designed for rapid acquisition LiveCell imaging in XYZ. The incubator allows control on temperature and CO2, and the motorized stage allows multiple-position imaging. Objectives can be mounted on a piezo for very fast z-acquisition. | |description=Our Zeiss Axiovert 200 4D-System is designed for rapid acquisition LiveCell imaging in XYZ. The incubator allows control on temperature and CO2, and the motorized stage allows multiple-position imaging. Objectives can be mounted on a piezo for very fast z-acquisition. | ||
|applications=- | |applications=- | ||
| Line 15: | Line 15: | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=motorized XYZ stage, z-Piezo, fluorescence, transmitted light with manual DIC | |microscope=motorized XYZ stage, z-Piezo, fluorescence, transmitted light with manual DIC | ||
| − | | | + | |obj1=Zeiss Plan-Apochromat 10x 0.45 |
| − | | | + | |obj2=Zeiss Plan-Apochromat 20x 0.8 Ph2 |
| − | | | + | |obj3=Zeiss EC Plan-Neofluar 40x 1.3 oil Ph3 |
| − | |ill1=Fluorescence ( | + | |obj4=Zeiss Plan-Apochromat 63x 1.4 Oil |
| − | |ill2=Fluorescence ( | + | |obj5=Zeiss C-Apochromat 63x 1.2 W |
| + | |obj6=Zeiss Plan-Apochromat 100x 1.4 Oil | ||
| + | |ill1=Fluorescence (DG4) | ||
| + | |ill2=Fluorescence (Lumencor Spectra X light engine) | ||
|ill3=Transmitted Light (Halogen) | |ill3=Transmitted Light (Halogen) | ||
|detection=CCD Coolsnap HQ (1392*1040, 6,45*6,45µm pixel) | |detection=CCD Coolsnap HQ (1392*1040, 6,45*6,45µm pixel) | ||
| − | |reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br>FITC : EX 480/30 ; BS 505 ; EM 535/40<br>DsRed : EX 545/30 ; BS 570 ; EM 620/60<br>Cy5 : EX 620/60 ; BS 660 ; EM 700/75<br>Dual Band CFP/YFP (EX 437/19 503/27 ; EM 469/37 549/67) available on request<br>Dual Band GFP/DsRed (EX 474/27 565/22 ; EM 524/52 619/68) available on request | + | |reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br> |
| + | FITC : EX 480/30 ; BS 505 ; EM 535/40<br> | ||
| + | DsRed : EX 545/30 ; BS 570 ; EM 620/60<br> | ||
| + | Cy5 : EX 620/60 ; BS 660 ; EM 700/75<br> | ||
| + | Dual Band CFP/YFP (EX 437/19 503/27 ; EM 469/37 549/67) available on request<br> | ||
| + | Dual Band GFP/DsRed (EX 474/27 565/22 ; EM 524/52 619/68) available on request<br> | ||
| + | Fura based Ca-imaging (EX 340/12 380/14 ; BS 405 ; EM 510/40) available on request <br> | ||
|features=sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging | |features=sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging | ||
| − | |software= | + | |software=MetaMorph |
| + | VisiView | ||
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
|link1=- | |link1=- | ||
|link2=- | |link2=- | ||
| − | |inv= | + | |inv=121524 |
|facility_image=Imaging@Biotec.jpg | |facility_image=Imaging@Biotec.jpg | ||
|logo_image=Logo_Biotec.jpg | |logo_image=Logo_Biotec.jpg | ||
Latest revision as of 11:29, 21 July 2015
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[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Zeiss - Axiovert 200M, Inverted stand, motorized XYZ stage, z-Piezo, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination | |||
| detection | CCD Coolsnap HQ (1392*1040, 6,45*6,45µm pixel) | ||
| reflectors | DAPI : EX 360/40 ; BS 400 ; EM 460/50 FITC : EX 480/30 ; BS 505 ; EM 535/40 | ||
| features | sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging | ||
| software | MetaMorph
VisiView"MetaMorph VisiView" cannot be used as a page name in this wiki. | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | 121524 |
