Live Cell AF6000, CRTD

From BioDIP
(Difference between revisions)
Jump to: navigation, search
 
(40 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 
{{Equipment
 
{{Equipment
| system-number       = 08
+
|system-number=BIO07
| system-name         = Leica AFLX6000 inverted
+
|system-name=Live Cell AF6000, CRTD
| category             = Wide-Field
+
|type=Microscope
| image                = Bioz08.jpg
+
|category=Wide-Field
| facility             = Imaging@Biotec
+
|facility=Imaging@BIOTEC/CRTD
| facility_image      = Logo_Imaging_at_Biotec_reduced.JPG
+
|building=CRTD
| building            = Biotec
+
|room=-1.123
| room                = 221
+
|Facility-logo=Imaging@Biotec.jpg
| description         = Our Leica AFLX6000 is designed for routine work and dedicated for LiveCell imaging in XYZ. The incubator allows control on temperature and CO2, and the motorized stage allows multiple-position imaging. The simple LASAF interface makes it straight forward to work with.
+
|Institute-logo=CRTD logo.PNG
| microscope           = inverted stand, motorized XYZ stage, fluorescence, transmitted light with automatic DIC
+
|description=Our Leica AFLX6000 is designed for routine work and dedicated for LiveCell imaging in XYZ. The incubator allows control on temperature and CO2, and the motorized stage allows multiple-position imaging. The simple LASAF interface makes it straight forward to work with. A double micromanipulator allows precise manipulation of the sample.
| objectives          = 10x/0.4 DIC<br>20x/0.7 DIC<br>40x/0.85 Corr<br>63x/1.3 Gly Corr 37°C (available on demand)
+
|applications=-
| illumination        = EL6000 (X-Cite)<br>Halogen
+
|image=Live Cell AF6000, CRTD.jpg
| detection           = CCD Leica DFC 350 FX R2 (1392*1040, 6,45*6,45µm pixel)
+
|stand-name=Leica - DMI6000
| reflectors           = DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30<br>TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP
+
|stand=Inverted
| features             = sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging
+
|microscope=inverted stand, motorized XYZ stage, fluorescence, transmitted light with automatic DIC
| incubation           = None
+
|obj1=Leica HCX PL FLUOTAR 5x 0.15
 +
|obj2=Leica HC PL APO 10x 0.4
 +
|obj3=Leica HC PL APO 20x 0.7
 +
|obj5=Leica HCX PL APO 40x 1.25-0.75 Oil
 +
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 +
|ill2=Transmitted Light (Halogen)
 +
|detection=CCD Leica DFC 350 FX R2 (1392*1040, 6,45*6,45µm pixel)<br>
 +
Photometrics Cascade (512*512, 16*16µm pixel)
 +
|reflectors=DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425<br>
 +
TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP<br>
 +
CFP : EX 436/20 ; BS 455 ; EM BP 480/40<br>
 +
YFP-T : EX 490/20 ; BS 515 ; EM 535/30<br>
 +
Analysator
 +
|features=sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, double micro-manipulator
 +
|software=-
 +
|incubation=Cage incubator (T+CO2)
 +
|link0=https://ifn.mpi-cbg.de/wiki/ifn/images/9/91/Leica_AF6000_Well_Plate_Acquisition.pdf well plate acquisition
 +
|link1=-
 +
|link2=-
 +
|inv=71924
 +
|facility_image=Imaging@Biotec.jpg
 +
|logo_image=LOGO_CRTD.jpg
 +
|objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/0.85 Corr<br>63x/1.3 Gly Corr 37°C (available on request)
 
}}
 
}}

Latest revision as of 13:41, 17 October 2014

Suitable for -.
Live Cell AF6000, CRTD.jpg


[edit] Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.123)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Leica - DMI6000, Inverted stand, inverted stand, motorized XYZ stage, fluorescence, transmitted light with automatic DIC
objectives
Leica HCX PL FLUOTAR 5x 0.15
Leica HC PL APO 10x 0.4
Leica HC PL APO 20x 0.7
Leica HCX PL APO 40x 1.25-0.75 Oil
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection CCD Leica DFC 350 FX R2 (1392*1040, 6,45*6,45µm pixel)

Photometrics Cascade (512*512, 16*16µm pixel)

reflectors DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425

TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP
CFP : EX 436/20 ; BS 455 ; EM BP 480/40
YFP-T : EX 490/20 ; BS 515 ; EM 535/30
Analysator

features sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, double micro-manipulator
software -
incubation Cage incubator (T+CO2)
links
  • [- beam path]
  • [ advanced system check]
inv.nr. 71924



Personal tools