MB1 - BioRad 2photon

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{{Equipment
 
{{Equipment
| system-number       = 05
+
|system-number=MPI-MB1
| system-name         = Bio-Rad 2-Photon
+
|system-name=Bio-Rad 2-Photon
| category             = Multiphoton Laser Scanning
+
|type=Not listed
| stand                = inverted
+
|category=Multiphoton Laser Scanning
| image                = MPI05.jpg
+
|facility=MPI-CBG LMF
| facility            = MPI-CBG LMF
+
|building=MPI-CBG
| facility_image      = logo-lmf.jpg
+
|room=038C
| building            = MPI-CBG
+
|Facility-logo=MPI-CBG-Lmf-logo-big.png
| room                = 038C
+
|Institute-logo=Logo MPI-CBG.jpg
| description         = Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser for multiphoton excitation.
+
|description=Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser for multiphoton excitation.
| applications         = imaging of thick specimens fast bleaching samples
+
|applications=imaging of thick specimens fast bleaching samples
| microscope          = Nikon TE300, manual XY stage, motorized Z-drive
+
|image=MPI05.jpg
| objectives          = 20x/0.75 Plan-Fluor<br>40x/0.8 W Nikon-Fluor<br>60x/1.2 WI Plan-Apo<br>63x/1.0 W Nikon-Fluor
+
|stand-name=Nikon - Eclipse TE300
| illumination        = Halogen<br>Prior Lumen 200 (metal halide)<br>multi-photon excitation: 700-980 nm Mira 900 (adjusted to 895 nm)<br>single-photon excitation: Argon laser (457, 476, 488, 514 nm), HeNe 543 nm laser
+
|stand=Inverted
| detection           = 2 non-descanned PMTs<br>2 confocal PMTs
+
|microscope=manual XY stage, motorized Z-drive
| reflectors           = EX 465-495, EM 515-555<br>EX 515-560, EM LP590<br>EX 505-550, EM 605-655
+
|obj1=Nikon Plan-ApochromatVC 20x 0.75 DIC
| features             = -
+
|obj2=Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC
| incubation           = mobile stage incubator
+
|obj3=Nikon Plan-Fluor 40x 0.75 DIC
| link1               = -
+
|obj4=Nikon Fluor 40x 0.8 W DIC
| link2               = [http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvpitmD6GEvaA beam path / configuration]
+
|obj5=Nikon Plan-Apochromat 60x 1.2 W
| inv                 = Nikon  Mikroscope : 403039 MIRA Laser System: 402408
+
|ill1=Transmitted Light (Halogen)
 +
|ill2=Fluorescence (Metal Halide, PRIOR, 200W)
 +
|ill3=Laser Multiphoton 700-980 nm
 +
|ill4=Laser Argon Multiline 458, 477, 488, 514 nm
 +
|ill5=Laser HeNe 543 nm
 +
|detection=2 non-descanned PMTs<br>2 confocal PMTs
 +
|reflectors=EX 465-495, EM 515-555<br>EX 515-560, EM LP590<br>EX 505-550, EM 605-655
 +
|features=-
 +
|software=lasersharp 2000
 +
|incubation=Stage incubator (T) + objective heater
 +
|link1=-
 +
|link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvpitmD6GEvaA
 +
|inv=Nikon  Microscope : 403254, MIRA Laser System: 402408   10.1.145.2
 +
|facility_image=logo-lmf.jpg
 +
|objectives=20x/0.75 Plan-Fluor<br>40x/0.8 W Nikon-Fluor<br>60x/1.2 WI Plan-Apo<br>63x/1.0 W Nikon-Fluor
 +
|illumination=Halogen<br>Prior Lumen 200 (metal halide)<br>multi-photon excitation: 700-980 nm Mira 900 (adjusted to 895 nm)<br>single-photon excitation: Argon laser (457, 476, 488, 514 nm), HeNe 543 nm laser
 
}}
 
}}

Latest revision as of 16:33, 3 February 2016

Suitable for imaging of thick specimens fast bleaching samples.
MPI05.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 038C)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Nikon - Eclipse TE300, Inverted stand, manual XY stage, motorized Z-drive
objectives
Nikon Plan-ApochromatVC 20x 0.75 DIC
Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC
Nikon Plan-Fluor 40x 0.75 DIC
Nikon Fluor 40x 0.8 W DIC
Nikon Plan-Apochromat 60x 1.2 W
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, PRIOR, 200W)
Laser Multiphoton 700-980 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser HeNe 543 nm
detection 2 non-descanned PMTs
2 confocal PMTs
reflectors EX 465-495, EM 515-555
EX 515-560, EM LP590
EX 505-550, EM 605-655
features -
software lasersharp 2000
incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [- laser power values]
inv.nr. Nikon Microscope : 403254, MIRA Laser System: 402408 10.1.145.2



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