MB1 - BioRad 2photon
From BioDIP
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=MPI-MB1 |
|system-name=Bio-Rad 2-Photon | |system-name=Bio-Rad 2-Photon | ||
| − | |type= | + | |type=Not listed |
|category=Multiphoton Laser Scanning | |category=Multiphoton Laser Scanning | ||
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
|room=038C | |room=038C | ||
| − | | | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
| + | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser for multiphoton excitation. | |description=Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser for multiphoton excitation. | ||
|applications=imaging of thick specimens fast bleaching samples | |applications=imaging of thick specimens fast bleaching samples | ||
|image=MPI05.jpg | |image=MPI05.jpg | ||
| + | |stand-name=Nikon - Eclipse TE300 | ||
|stand=Inverted | |stand=Inverted | ||
| − | |microscope= | + | |microscope=manual XY stage, motorized Z-drive |
| − | |obj1=- | + | |obj1=Nikon Plan-ApochromatVC 20x 0.75 DIC |
| − | |obj2=- | + | |obj2=Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC |
| − | |obj3=- | + | |obj3=Nikon Plan-Fluor 40x 0.75 DIC |
| − | |obj4= | + | |obj4=Nikon Fluor 40x 0.8 W DIC |
| − | |obj5=- | + | |obj5=Nikon Plan-Apochromat 60x 1.2 W |
| − | + | ||
| − | + | ||
| − | + | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
| − | |ill2=Fluorescence (Metal Halide) | + | |ill2=Fluorescence (Metal Halide, PRIOR, 200W) |
|ill3=Laser Multiphoton 700-980 nm | |ill3=Laser Multiphoton 700-980 nm | ||
|ill4=Laser Argon Multiline 458, 477, 488, 514 nm | |ill4=Laser Argon Multiline 458, 477, 488, 514 nm | ||
|ill5=Laser HeNe 543 nm | |ill5=Laser HeNe 543 nm | ||
| − | |||
| − | |||
| − | |||
|detection=2 non-descanned PMTs<br>2 confocal PMTs | |detection=2 non-descanned PMTs<br>2 confocal PMTs | ||
|reflectors=EX 465-495, EM 515-555<br>EX 515-560, EM LP590<br>EX 505-550, EM 605-655 | |reflectors=EX 465-495, EM 515-555<br>EX 515-560, EM LP590<br>EX 505-550, EM 605-655 | ||
|features=- | |features=- | ||
| − | |incubation= | + | |software=lasersharp 2000 |
| + | |incubation=Stage incubator (T) + objective heater | ||
|link1=- | |link1=- | ||
|link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvpitmD6GEvaA | |link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvpitmD6GEvaA | ||
| − | |inv=Nikon Microscope : | + | |inv=Nikon Microscope : 403254, MIRA Laser System: 402408 10.1.145.2 |
| + | |facility_image=logo-lmf.jpg | ||
|objectives=20x/0.75 Plan-Fluor<br>40x/0.8 W Nikon-Fluor<br>60x/1.2 WI Plan-Apo<br>63x/1.0 W Nikon-Fluor | |objectives=20x/0.75 Plan-Fluor<br>40x/0.8 W Nikon-Fluor<br>60x/1.2 WI Plan-Apo<br>63x/1.0 W Nikon-Fluor | ||
|illumination=Halogen<br>Prior Lumen 200 (metal halide)<br>multi-photon excitation: 700-980 nm Mira 900 (adjusted to 895 nm)<br>single-photon excitation: Argon laser (457, 476, 488, 514 nm), HeNe 543 nm laser | |illumination=Halogen<br>Prior Lumen 200 (metal halide)<br>multi-photon excitation: 700-980 nm Mira 900 (adjusted to 895 nm)<br>single-photon excitation: Argon laser (457, 476, 488, 514 nm), HeNe 543 nm laser | ||
}} | }} | ||
Latest revision as of 16:33, 3 February 2016
|
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Nikon - Eclipse TE300, Inverted stand, manual XY stage, motorized Z-drive | ||
| objectives | |||
| illumination | |||
| detection | 2 non-descanned PMTs 2 confocal PMTs | ||
| reflectors | EX 465-495, EM 515-555 EX 515-560, EM LP590 EX 505-550, EM 605-655 | ||
| features | - | ||
| software | lasersharp 2000 | ||
| incubation | Stage incubator (T) + objective heater | ||
| links |
| ||
| inv.nr. | Nikon Microscope : 403254, MIRA Laser System: 402408 10.1.145.2 |
