MB1 - BioRad 2photon

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|link1=-
 
|link1=-
 
|link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvpitmD6GEvaA
 
|link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvpitmD6GEvaA
|inv=Nikon  Microscope : 403039 MIRA Laser System: 402408    10.1.145.2
+
|inv=Nikon  Microscope : 403254, MIRA Laser System: 402408    10.1.145.2
 
|facility_image=logo-lmf.jpg
 
|facility_image=logo-lmf.jpg
 
|objectives=20x/0.75 Plan-Fluor<br>40x/0.8 W Nikon-Fluor<br>60x/1.2 WI Plan-Apo<br>63x/1.0 W Nikon-Fluor
 
|objectives=20x/0.75 Plan-Fluor<br>40x/0.8 W Nikon-Fluor<br>60x/1.2 WI Plan-Apo<br>63x/1.0 W Nikon-Fluor
 
|illumination=Halogen<br>Prior Lumen 200 (metal halide)<br>multi-photon excitation: 700-980 nm Mira 900 (adjusted to 895 nm)<br>single-photon excitation: Argon laser (457, 476, 488, 514 nm), HeNe 543 nm laser
 
|illumination=Halogen<br>Prior Lumen 200 (metal halide)<br>multi-photon excitation: 700-980 nm Mira 900 (adjusted to 895 nm)<br>single-photon excitation: Argon laser (457, 476, 488, 514 nm), HeNe 543 nm laser
 
}}
 
}}

Revision as of 14:13, 23 September 2011

Suitable for imaging of thick specimens fast bleaching samples.
MPI05.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038C)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Nikon - Eclipse TE300, Inverted stand, manual XY stage, motorized Z-drive
objectives
Nikon Planfluor 20x/0.75 MImm
Nikon Fluor 40x/0.8 W DIC
Nikon Sfluor 40x/1.3 Oil DIC
Nikon PlanApo 20x/0.75 DIC
Nikon PlanApo 60x/1.2 WI 0.15-0.18
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, PRIOR, 200W)
Laser Multiphoton 700-980 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser HeNe 543 nm
detection 2 non-descanned PMTs
2 confocal PMTs
reflectors EX 465-495, EM 515-555
EX 515-560, EM LP590
EX 505-550, EM 605-655
features -
software lasersharp 2000
incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [- laser power values]
inv.nr. Nikon Microscope : 403254, MIRA Laser System: 402408 10.1.145.2



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