View source for ML1 - Leica 2photon in-vivo

{{Equipment
|system-number=MPI- ML1
|system-name=ML1 - Leica TCS SP8 MP
|type=Microscope
|category=Multiphoton Laser Scanning
|facility=MPI-CBG LMF
|building=MPI-CBG
|room=BMS 009
|Facility-logo=MPI-CBG-Lmf-logo-big.png
|Institute-logo=Logo MPI-CBG.jpg
|description=Multiphoton Laser Scanning Microscope equipped with an 8kHz resonant / galvo tandem scanner. Multiphoton excitation is possible by two pulsed NIR laser lines: one tunable from 680nm-1300nm, one fixed at 1040nm, and detected by four non-descanned detectors (two HyDs and two PMTs). It can also be used as a single photon confocal system with five spectral detectors (four HyDs and one PMT).
|applications=intravital / in vivo imaging and thick specimens. Fast imaging of sensitive samples, spectral imaging, tile scan and multi-position scan.
|image=ML1 - Leica TCS SP8 MP.jpg
|stand-name=Leica - DMI6000
|stand=Inverted
|microscope=XY motorized, motorized Z-focus, Super-Z Galvo Table
|obj1=Leica HC CS PL APO 10x 0.4
|obj2=Leica HC CS PL APO 20x 0.7
|obj3=Leica HC CS2 PL APO 20x 0.75 Imm
|obj4=Leica HCX IRAPO L 25x 0.95 W
|obj5=Leica HC PL IRAPO 40x 1.1 W
|obj6=Leica HC CS2 PL APO 63x 1.3 Glyc 23/37°C
|ill1=Laser Diode 405 nm
|ill2=Laser Solid State 488 nm
|ill3=Laser Solid State 552 nm
|ill4=Laser Solid State 638 nm
|ill5=Laser Multiphoton Spectra Physics InSight Deep See Dual
|ill6=Fluorescence (Metal Halide)
|detection=Confocal: spectral detector with five detectors: four hybrid detectors and one PMT<br>
Multiphoton: four non-descanned detectors: two hybrid detectors and two PMTs
|reflectors=DAPI ET <br>
GFP/RFP
|features=resonant scanner, hybrid detectors, simultaneous imaging, sequential imaging, line scan, fast point scan, fast Z stacks, time-series, scan zoom and rotation, tile scan, multi position scan, spectral scan
|software=LAS X
|incubation=Stage incubator (T+CO2)
|link1=https://docs.google.com/spreadsheets/d/1tAI1MKo-1uTyWYIhktkN9AZ6E3WRduNSiiUcFAq20LY/pubhtml?gid=0&single=true
|inv=0
}}