ML1 - Leica 2photon in-vivo

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m (adjusting system id to the form of LZ2)
(update software version, add link to laser power values)
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GFP/RFP
 
GFP/RFP
 
|features=resonant scanner, hybrid detectors, simultaneous imaging, sequential imaging, line scan, fast point scan, fast Z stacks, time-series, scan zoom and rotation, tile scan, multi position scan, spectral scan
 
|features=resonant scanner, hybrid detectors, simultaneous imaging, sequential imaging, line scan, fast point scan, fast Z stacks, time-series, scan zoom and rotation, tile scan, multi position scan, spectral scan
|software=LASF AF3
+
|software=LASF X
 
|incubation=Stage incubator (T+CO2)
 
|incubation=Stage incubator (T+CO2)
 +
|link1=https://docs.google.com/spreadsheets/d/1tAI1MKo-1uTyWYIhktkN9AZ6E3WRduNSiiUcFAq20LY/edit?usp=sharing
 
|inv=0
 
|inv=0
 
}}
 
}}

Revision as of 18:43, 3 February 2016

Suitable for intravital / in vivo imaging and thick specimens. Fast imaging of sensitive samples, spectral imaging, tile scan and multi-position scan..
ML1 - Leica TCS SP8 MP.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, BMS 009)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Leica - DMI6000, Inverted stand, XY motorized, motorized Z-focus, Super-Z Galvo Table
objectives
Leica HC CS PL APO 10x 0.4
Leica HC CS PL APO 20x 0.7
Leica HC CS2 PL APO 20x 0.75 Imm
Leica HCX IRAPO L 25x 0.95 W
Leica HC PL IRAPO 40x 1.1 W
Leica HC CS2 PL APO 63x 1.3 Glyc 23/37°C
illumination
Laser Diode 405 nm
Laser Solid State 488 nm
Laser Solid State 552 nm
Laser Solid State 638 nm
Laser Multiphoton Spectra Physics InSight Deep See Dual
Fluorescence (Metal Halide)
detection Confocal: spectral detector with five detectors: four hybrid detectors and one PMT

Multiphoton: four non-descanned detectors: two hybrid detectors and two PMTs

reflectors DAPI ET

GFP/RFP

features resonant scanner, hybrid detectors, simultaneous imaging, sequential imaging, line scan, fast point scan, fast Z stacks, time-series, scan zoom and rotation, tile scan, multi position scan, spectral scan
software LASF X
incubation Stage incubator (T+CO2)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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