ML1 - Leica 2photon in-vivo

From BioDIP
(Difference between revisions)
Jump to: navigation, search
Line 9: Line 9:
 
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 
|Institute-logo=Logo MPI-CBG.jpg
 
|Institute-logo=Logo MPI-CBG.jpg
|description=*Multiphoton Laser Scanning Microscope equipped with an 8kHz resonant / galvo tandem scanner. Multiphoton excitation is possible by two pulsed NIR laser lines: one tunable from 680nm-1300nm, one fixed at 1040nm, and detected by four non-descanned detectors (two HyDs and two PMTs). It can also be used as a single photon confocal system with five spectral detectors (four HyDs and one PMT).
+
|description=Multiphoton Laser Scanning Microscope equipped with an 8kHz resonant / galvo tandem scanner. Multiphoton excitation is possible by two pulsed NIR laser lines: one tunable from 680nm-1300nm, one fixed at 1040nm, and detected by four non-descanned detectors (two HyDs and two PMTs). It can also be used as a single photon confocal system with five spectral detectors (four HyDs and one PMT).
 
|applications=intravital / in vivo imaging and thick specimens. Fast imaging of sensitive samples, spectral imaging, tile scan and multi-position scan.
 
|applications=intravital / in vivo imaging and thick specimens. Fast imaging of sensitive samples, spectral imaging, tile scan and multi-position scan.
 
|image=ML1 - Leica TCS SP8 MP.jpg
 
|image=ML1 - Leica TCS SP8 MP.jpg

Revision as of 19:15, 3 February 2016

Suitable for intravital / in vivo imaging and thick specimens. Fast imaging of sensitive samples, spectral imaging, tile scan and multi-position scan..
ML1 - Leica TCS SP8 MP.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, BMS 009)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Leica - DMI6000, Inverted stand, XY motorized, motorized Z-focus, Super-Z Galvo Table
objectives
Leica HC CS PL APO 10x 0.4
Leica HC CS PL APO 20x 0.7
Leica HC CS2 PL APO 20x 0.75 Imm
Leica HCX IRAPO L 25x 0.95 W
Leica HC PL IRAPO 40x 1.1 W
Leica HC CS2 PL APO 63x 1.3 Glyc 23/37°C
illumination
Laser Diode 405 nm
Laser Solid State 488 nm
Laser Solid State 552 nm
Laser Solid State 638 nm
Laser Multiphoton Spectra Physics InSight Deep See Dual
Fluorescence (Metal Halide)
detection Confocal: spectral detector with five detectors: four hybrid detectors and one PMT

Multiphoton: four non-descanned detectors: two hybrid detectors and two PMTs

reflectors DAPI ET

GFP/RFP

features resonant scanner, hybrid detectors, simultaneous imaging, sequential imaging, line scan, fast point scan, fast Z stacks, time-series, scan zoom and rotation, tile scan, multi position scan, spectral scan
software LAS X
incubation Stage incubator (T+CO2)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



Personal tools