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− | == How to start the Olympus FV1000 == | + | == How to switch off the Zeiss 2photon upright == |
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| === Hardware === | | === Hardware === |
− | [[image:mpi09_561.jpg|200x100px]]
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− | [[image:mpi09_argon-633.jpg|200x100px]]
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− | [[image:mpi09_powermanager.jpg|200x100px]]
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− | [[image:mpi09_stagecontroller.jpg|200x100px]]
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− | # Switch on lasers you need:
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− | #* 633nm laser: turn key
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− | #* Argon laser: switch button, then turn the key
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− | #* 561nm laser: press the green button
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− | # Switch on the motorised stage
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− | # Switch on the rest of the system using the power manager below the monitors. Think about what components you really need (otherwise leave them switched off):
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− | #* LD405 ... 405nm imaging laser
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− | #* 405nm cutter ... bleaching/ ablation laser
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− | === Software Initiation===
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− | [[image:mpi09_sw-icon.jpg|200x100px]] [[image:mpi09_sw-login.jpg|200x100px]]
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− | # Start software and log in as lmf user.
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| === Basic Acquisition Setup === | | === Basic Acquisition Setup === |
− | [[image:mpi09_sw-dyelist.jpg|200x100px]] [[image:mpi09_sw-acquisition.jpg|200x100px]] [[image:mpi09_sw-explorer.jpg|200x100px]]
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− | # If applicable, use the Olympus Dye list to help you set up the beam path settings.
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− | # Set up the aquisition parameters according to your sample and needs. Once you have an image, use the HV and offset function to adjust for saturation and background.
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− | # You might want to reuse parameters from an old image using the Explorer. Look for your image data, right mouse click on it and choose “Reload aquisition parameters”
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| === Basic Troubleshooting === | | === Basic Troubleshooting === |
− | [[image:mpi09_trouble-shutter.jpg|200x100px]] [[image:mpi09_trouble-sim.jpg|200x100px]]
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− | * PROBLEM: No light at the object plane!
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− | ** CHECK: excitation shutter of turret open??
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− | * PROBLEM: No 405nm imaging laser at the object plane!
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− | ** CHECK: SIM function disabled??
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| [[category:hardware]] | | [[category:hardware]] |
| [[category:MPI-MZ1 manuals]] | | [[category:MPI-MZ1 manuals]] |