MPI-MZ1 - Zeiss 2photon upright - startup & shutdown

How to start the Olympus FV1000

Hardware

1. Switch on lasers you need:
   • 633nm laser: turn key
   • Argon laser: switch button, then turn the key
   • 561nm laser: press the green button
2. Switch on the motorised stage
3. Switch on the rest of the system using the power manager below the monitors. Think about what components you really need (otherwise leave them switched off):
   • LD405 ... 405nm imaging laser
   • 405nm cutter ... bleaching/ ablation laser

Software Initiation

1. Start software and log in as lmf user.

Basic Acquisition Setup

1. If applicable, use the Olympus Dye list to help you set up the beam path settings.
2. Set up the aquisition parameters according to your sample and needs. Once you have an image, use the HV and offset function to adjust for saturation and background.
3. You might want to reuse parameters from an old image using the Explorer. Look for your image data, right mouse click on it and choose “Reload aquisition parameters”

Basic Troubleshooting

• PROBLEM: No light at the object plane!
  • CHECK: excitation shutter of turret open??
• PROBLEM: No 405nm imaging laser at the object plane!
  • CHECK: SIM function disabled??