MPI-TDS1

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Line 21: Line 21:
 
|ill2=Laser DPSS 488 nm
 
|ill2=Laser DPSS 488 nm
 
|ill3=Laser DPSS 561 nm
 
|ill3=Laser DPSS 561 nm
−
|ill4=Laser DPSS 639nm
+
|ill4=Laser Diode 635 nm
−
|detection=- 3 CCD cameras
+
|detection=- 3 CCD camera with 1.3 mega pixel – peltier-cooled – 12 bit resolution
 
|reflectors=- Filters: various filters to detect a wide spectrum from Alexa405/DAPI to Alexa647/DRAQ5
 
|reflectors=- Filters: various filters to detect a wide spectrum from Alexa405/DAPI to Alexa647/DRAQ5
 
|features=- plate robot : Twister II
 
|features=- plate robot : Twister II

Revision as of 16:56, 8 June 2011

The on-the-fly image analysis software Acapella runs paralell on 3 CIAs"The Opera QEHS is a fully automated confocal microscope, using the Nipkow-disc. It has multiple high-power lasers and is enabled to do multi colour imaging. It has a laser-autofocus and an on-board dispensing unit.It's equipped with 3 cameras having a minimal exposure time of 40ms and can produce up to 100.000 multicolour images per day. The on-the-fly image analysis software Acapella runs paralell on 3 CIAs" cannot be used as a page name in this wiki.

Suitable for Automated high-resolution, high-throughput imaging of thin-bottom microtiter plates (96well, 384well, 1536well)..
MPI-TDS1-Opera.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] TDS"TDS" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. ("MPI-CBG TDS" is not in the list of possible values (BIOTEC, CRTD, DZNE, MTZ, MPI-CBG) for this property., (MPI-CBG, 127))

Booking

https://java-srv1.mpi-cbg.de/cart/

Details

microscope Olympus - IX70, Inverted stand, -
objectives
Olympus UPlanApo 60x 1.2 W
Olympus UPlanApo 40x 0.85
Olympus UPlanApo 20x 0.7
Olympus UPlanApo 10x 0.4
illumination
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser Diode 635 nm
detection - 3 CCD camera with 1.3 mega pixel – peltier-cooled – 12 bit resolution
reflectors - Filters: various filters to detect a wide spectrum from Alexa405/DAPI to Alexa647/DRAQ5
features - plate robot : Twister II
software - EvoShell

- Acapella (image analysis)"- EvoShell - Acapella (image analysis)" cannot be used as a page name in this wiki.

incubation Not available
links
  • manual
  • [405nm (50mW), 488nm (200mW), 561nm (40mW), 635nm (2x25mW) laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0


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