MPI-TDS1

From BioDIP
(Difference between revisions)
Jump to: navigation, search
 
(10 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 
{{Equipment
 
{{Equipment
 
|system-number=MPI-TDS1
 
|system-number=MPI-TDS1
|system-name=PerkinElmer Opera
+
|system-name=Perkin Elmer Opera
 
|type=Microscope
 
|type=Microscope
 
|category=Real-Time Confocal
 
|category=Real-Time Confocal
|facility=TDS
+
|facility=MPI-CBG TDS
|building=MPI-CBG TDS
+
|building=MPI-CBG
 
|room=(MPI-CBG, 127)
 
|room=(MPI-CBG, 127)
 +
|Facility-logo=TDS-logo.png
 +
|Institute-logo=Logo MPI-CBG.jpg
 
|description=The Opera QEHS is a fully automated confocal microscope, using the Nipkow-disc. It has multiple high-power lasers and is enabled to do multi colour imaging. It has a laser-autofocus and an on-board dispensing unit.It's equipped with 3 cameras having a minimal exposure time of 40ms and can produce up to 100.000 multicolour images per day.
 
|description=The Opera QEHS is a fully automated confocal microscope, using the Nipkow-disc. It has multiple high-power lasers and is enabled to do multi colour imaging. It has a laser-autofocus and an on-board dispensing unit.It's equipped with 3 cameras having a minimal exposure time of 40ms and can produce up to 100.000 multicolour images per day.
 
The on-the-fly image analysis software Acapella runs paralell on 3 CIAs
 
The on-the-fly image analysis software Acapella runs paralell on 3 CIAs
 
|applications=Automated high-resolution, high-throughput imaging of thin-bottom microtiter plates (96well, 384well, 1536well).
 
|applications=Automated high-resolution, high-throughput imaging of thin-bottom microtiter plates (96well, 384well, 1536well).
 
|image=MPI-TDS1-Opera.jpg
 
|image=MPI-TDS1-Opera.jpg
|stand-name=Olympus - IX70
+
|stand-name=TILL Photonics - iMIC
 
|stand=Inverted
 
|stand=Inverted
 
|microscope=-
 
|microscope=-
|obj1=Olympus UPlanApo 60x 1.2 W
+
|obj1=Olympus UPlanSApo 10x 0.4
|obj2=Olympus UPlanApo 40x 0.85
+
|obj2=Olympus UApo 20x W3 0.7 W
|obj3=Olympus UPlanApo 20x 0.7
+
|obj3=Olympus PlanApo 40x WLSM-2 0.9 W
|obj4=Olympus UPlanApo 10x 0.4
+
|obj4=Olympus UPlanApo 60x 1.2 W
 
|ill1=Laser Diode 405 nm
 
|ill1=Laser Diode 405 nm
 
|ill2=Laser DPSS 488 nm
 
|ill2=Laser DPSS 488 nm
 
|ill3=Laser DPSS 561 nm
 
|ill3=Laser DPSS 561 nm
|ill4=Laser DPSS 639nm
+
|ill4=Laser Diode 635 nm
|detection=- 3 CCD cameras
+
|detection=- 3x CCD cameras with 1.3 mega pixel – peltier-cooled – 12 bit resolution
 
|reflectors=- Filters: various filters to detect a wide spectrum from Alexa405/DAPI to Alexa647/DRAQ5
 
|reflectors=- Filters: various filters to detect a wide spectrum from Alexa405/DAPI to Alexa647/DRAQ5
 
|features=- plate robot : Twister II
 
|features=- plate robot : Twister II
Line 30: Line 32:
 
|link0=https://zope.mpi-cbg.de/intranet/services/tds/manuals/Opera%20QEHS%20Hardware%20Manual.pdf
 
|link0=https://zope.mpi-cbg.de/intranet/services/tds/manuals/Opera%20QEHS%20Hardware%20Manual.pdf
 
|link1=405nm (50mW), 488nm (200mW), 561nm (40mW), 635nm (2x25mW)
 
|link1=405nm (50mW), 488nm (200mW), 561nm (40mW), 635nm (2x25mW)
 +
|link2=https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:MPI-CBG_TDS1_Opera_BeamPath.png
 +
|link3=https://zope.mpi-cbg.de/intranet/services/tds/service-facility/instruments/evotec-opera2
 
|inv=0
 
|inv=0
 
}}
 
}}

Latest revision as of 12:21, 18 November 2013

The on-the-fly image analysis software Acapella runs paralell on 3 CIAs"The Opera QEHS is a fully automated confocal microscope, using the Nipkow-disc. It has multiple high-power lasers and is enabled to do multi colour imaging. It has a laser-autofocus and an on-board dispensing unit.It's equipped with 3 cameras having a minimal exposure time of 40ms and can produce up to 100.000 multicolour images per day. The on-the-fly image analysis software Acapella runs paralell on 3 CIAs" cannot be used as a page name in this wiki.

Suitable for Automated high-resolution, high-throughput imaging of thin-bottom microtiter plates (96well, 384well, 1536well)..
MPI-TDS1-Opera.jpg


[edit] Directions

Logo MPI-CBG.jpg
TDS-logo.pngMPI-CBG TDS (MPI-CBG, (MPI-CBG, 127))

[edit] Booking

https://java-srv1.mpi-cbg.de/cart/

[edit] Details

microscope TILL Photonics - iMIC, Inverted stand, -
objectives
Olympus UPlanSApo 10x 0.4
Olympus UApo 20x W3 0.7 W
Olympus PlanApo 40x WLSM-2 0.9 W
Olympus UPlanApo 60x 1.2 W
illumination
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser Diode 635 nm
detection - 3x CCD cameras with 1.3 mega pixel – peltier-cooled – 12 bit resolution
reflectors - Filters: various filters to detect a wide spectrum from Alexa405/DAPI to Alexa647/DRAQ5
features - plate robot : Twister II
software - EvoShell

- Acapella (image analysis)"- EvoShell - Acapella (image analysis)" cannot be used as a page name in this wiki.

incubation Not available
links
  • manual
  • [405nm (50mW), 488nm (200mW), 561nm (40mW), 635nm (2x25mW) laser power values]
inv.nr. 0



Personal tools