MTZ01 - Zeiss LSM 510
From BioDIP
(Difference between revisions)
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| objectives = 10x/0.3<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo | | objectives = 10x/0.3<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo | ||
| excitation = HBO<br>405nm Diode laser<br>458, 488, 514nm Argon laser<br>561nm DPSS laser<br>633nm HeNe laser | | excitation = HBO<br>405nm Diode laser<br>458, 488, 514nm Argon laser<br>561nm DPSS laser<br>633nm HeNe laser | ||
| − | | detection = point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | + | | detection = point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector<br>[http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/systems/01_zeiss_lsm_510/01_zeiss_lsm_510_filterconfig.pdf scan-head filter configuration] |
| reflectors = DAPI (EX 350/50, EM 460/50)<br>GFP (EX 472/30, EM 520/35)<br>RFP (EX 546/12, EM LP590) | | reflectors = DAPI (EX 350/50, EM 460/50)<br>GFP (EX 472/30, EM 520/35)<br>RFP (EX 546/12, EM LP590) | ||
| features = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation | | features = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation | ||
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Revision as of 11:59, 16 June 2008
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://cgc.med.tu-dresden.de/phpScheduleIt
Details
| microscope | , stand, inverted stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
| objectives |
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| illumination |
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| detection | [[detection::point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector scan-head filter configuration]] | ||
| reflectors | DAPI (EX 350/50, EM 460/50) GFP (EX 472/30, EM 520/35) RFP (EX 546/12, EM LP590) | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation | ||
| software | |||
| incubation | |||
| links |
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| inv.nr. |
[[Category:{{{type}}}]]
