MTZ01 - Zeiss LSM 510

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[[Image:Logo-mtzimaging.jpg|thumb]]
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{{Equipment
 
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|system-number=MTZ01
[[Image:MTZ01.jpg|thumb]]
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|system-name=Zeiss LSM 510
 
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|type=Microscope
== [01] Zeiss LSM 510 ==
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|category=Laser Scanning Confocal
MTZ, B20.001B
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|facility=MTZ Imaging
 
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|building=MTZ
* Our Zeiss LSM 510 is designed for routine work, mainly with fixed samples. It is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
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|room=B20.001B
; microscope
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|Facility-logo=Logo mtz 3.png
* stand: inverted
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|Institute-logo=Logo Medizin.png
* stage: manual XY
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|description=This [[Zeiss LSM 510]] [[confocal]] microscope is designed for routine work. The laser lines are well distributed over the spectrum. Due to the conventional setup, it offers straight forward working conditions.
* Z-drive: motorized objective turret
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|applications=routine work with fixed samples, high resolution imaging in XYZ and common dye combinations
* objectives: 10x/0.3 AIR, 25x/0.8 IMM CORR, 40x/1.3 OIL, 63x/1.4 APO OIL, 63x/1.2 APO W
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|image=MTZ01.jpg
* constrasting techniques: DIC (manual), fluorescence
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|stand-name=Zeiss - Axiovert 200M
; excitation
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|stand=Inverted
* laser (nm): 405, 458, 488, 514, 561, 633
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|microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
; detection
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|obj1=Zeiss EC Plan-Neofluar 10x 0.3
* type: point scanner, 3 confocal PMTs, T-PMT
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|obj2=Zeiss LCI Plan-Neofluar 25x 0.8 Imm Corr
* excitation/emission splitting: dichromatic mirrors
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|obj3=Zeiss EC Plan-Neofluar 40x 1.3 Oil
* detection range selection: emission filters
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|obj4=Zeiss Plan-Apochromat 63x 1.4 Oil
* pinhole: for each detector
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|obj5=Zeiss C-Apochromat 63x 1.2 W
; software features
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|ill1=Transmitted Light (Halogen)
* simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing
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|ill2=Fluorescence (HBO)
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|ill3=Laser Diode 405 nm
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|ill4=Laser Argon Multiline 458, 477, 488, 514 nm
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|ill5=Laser DPSS 561 nm
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|ill6=Laser HeNe 633 nm
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|detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
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|reflectors=EX 350/50, EM 460/50<br>EX 472/30, EM 520/35<br>EX 546/12, EM LP590
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|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation
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|incubation=Not available
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|link1=http://spreadsheets.google.com/pub?key=pFf3a5iBejjAwSkUV9oO6fw
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|link2=https://spreadsheets.google.com/pub?key=pMZJXCgd5HNumbQenUfeIeA
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|inv=-
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|facility_image=Logo-mtzimaging.jpg
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|objectives=10x/0.3<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo
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|illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 488, 514 nm Argon laser<br>561 nm DPSS laser<br>633 nm HeNe laser
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}}

Latest revision as of 11:40, 14 November 2013

Suitable for routine work with fixed samples, high resolution imaging in XYZ and common dye combinations.
MTZ01.jpg


[edit] Directions

Logo Medizin.png
Logo mtz 3.pngMTZ Imaging (MTZ, B20.001B)

[edit] Booking

https://cgc.med.tu-dresden.de/phpScheduleIt

[edit] Details

microscope Zeiss - Axiovert 200M, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
objectives
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss LCI Plan-Neofluar 25x 0.8 Imm Corr
Zeiss EC Plan-Neofluar 40x 1.3 Oil
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss C-Apochromat 63x 1.2 W
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
detection point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
reflectors EX 350/50, EM 460/50
EX 472/30, EM 520/35
EX 546/12, EM LP590
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation
software
incubation Not available
links
inv.nr. -