MTZ01 - Zeiss LSM 510
From BioDIP
(Difference between revisions)
| Line 17: | Line 17: | ||
* Z-drive: motorized objective turret | * Z-drive: motorized objective turret | ||
* objectives: 10x/0.3 AIR, 25x/0.8 IMM CORR, 40x/1.3 OIL, 63x/1.4 APO OIL, 63x/1.2 APO W | * objectives: 10x/0.3 AIR, 25x/0.8 IMM CORR, 40x/1.3 OIL, 63x/1.4 APO OIL, 63x/1.2 APO W | ||
| + | * constrasting techniques: DIC (manual), fluorescence | ||
| + | |||
| + | === excitation === | ||
| + | |||
| + | * laser (nm): 405, 458, 488, 514, 561, 633 | ||
| + | |||
| + | === detection === | ||
| + | |||
| + | * type: point scanner, 3 confocal PMTs, T-PMT | ||
| + | * excitation/emission splitting: dichromatic mirrors | ||
| + | * detection range selection: emission filters | ||
| + | * pinhole: for each detector | ||
Revision as of 11:09, 3 June 2008
Contents |
[01] Zeiss LSM 510
MTZ, B20.001B
summary
Our Zeiss LSM 510 is designed for routine work, mainly with fixed samples. It is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
microscope
- stand: inverted
- stage: manual XY
- Z-drive: motorized objective turret
- objectives: 10x/0.3 AIR, 25x/0.8 IMM CORR, 40x/1.3 OIL, 63x/1.4 APO OIL, 63x/1.2 APO W
- constrasting techniques: DIC (manual), fluorescence
excitation
- laser (nm): 405, 458, 488, 514, 561, 633
detection
- type: point scanner, 3 confocal PMTs, T-PMT
- excitation/emission splitting: dichromatic mirrors
- detection range selection: emission filters
- pinhole: for each detector
