MTZ01 - Zeiss LSM 510
From BioDIP
(Difference between revisions)
| Line 22: | Line 22: | ||
* detection range selection: emission filters | * detection range selection: emission filters | ||
* pinhole: for each detector | * pinhole: for each detector | ||
| + | ; software | ||
| + | *simultaneous imaging | ||
| + | *sequential imaging | ||
| + | *line scan, point scan | ||
| + | *Z stacks | ||
| + | *time-series | ||
| + | *advanced time-series | ||
| + | *averaging, summarizing | ||
Revision as of 11:22, 3 June 2008
[01] Zeiss LSM 510
MTZ, B20.001B
- summary
- Our Zeiss LSM 510 is designed for routine work, mainly with fixed samples. It is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
- microscope
- stand: inverted
- stage: manual XY
- Z-drive: motorized objective turret
- objectives: 10x/0.3 AIR, 25x/0.8 IMM CORR, 40x/1.3 OIL, 63x/1.4 APO OIL, 63x/1.2 APO W
- constrasting techniques: DIC (manual), fluorescence
- excitation
- laser (nm): 405, 458, 488, 514, 561, 633
- detection
- type: point scanner, 3 confocal PMTs, T-PMT
- excitation/emission splitting: dichromatic mirrors
- detection range selection: emission filters
- pinhole: for each detector
- software
- simultaneous imaging
- sequential imaging
- line scan, point scan
- Z stacks
- time-series
- advanced time-series
- averaging, summarizing
