MTZ01 - Zeiss LSM 510

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[01] Zeiss LSM 510

MTZ, B20.001B

summary
  • Our Zeiss LSM 510 is designed for routine work, mainly with fixed samples. It is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
microscope
  • stand: inverted
  • stage: manual XY
  • Z-drive: motorized objective turret
  • objectives: 10x/0.3 AIR, 25x/0.8 IMM CORR, 40x/1.3 OIL, 63x/1.4 APO OIL, 63x/1.2 APO W
  • constrasting techniques: DIC (manual), fluorescence
excitation
  • laser (nm): 405, 458, 488, 514, 561, 633
detection
  • type: point scanner, 3 confocal PMTs, T-PMT
  • excitation/emission splitting: dichromatic mirrors
  • detection range selection: emission filters
  • pinhole: for each detector
software
  • simultaneous imaging
  • sequential imaging
  • line scan, point scan
  • Z stacks
  • time-series
  • advanced time-series
  • averaging, summarizing
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