MTZ02 - Leica TCS SP5
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| building = MTZ | | building = MTZ | ||
| room = B20.001C | | room = B20.001C | ||
− | | description = | + | | description = This [[Leica TCS SP5]] [[confocal]] microscope is specialized for Life Cell Imaging, FRET and FRAP. The selected laser equipment makes it suitable for i.e. GFP+mCherry and CFP+YFP. Modules for time-series and bleaching experiments allow to run also more complex experiments, but one can also perform high resolution scans with fixed and embedded samples. The spectral detection system offers flexibility in the combination of dyes. Motorized stage and DIC optics offer the possibility to perform semi-automated microscopy. |
| microscope = inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC | | microscope = inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC | ||
| objectives = 10x/0.3<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo | | objectives = 10x/0.3<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo |
Revision as of 15:03, 27 June 2008
Directions
[[image:|150px|text-bottom|right]]
Booking
https://cgc.med.tu-dresden.de/phpScheduleIt
Details
microscope | , inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC | ||
objectives |
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illumination |
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detection | point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel) | ||
reflectors | DAPI (EX 360/40, EM LP425) GFP (EX 470/40, EM LP515) RFP (EX 538/45, EM LP590) | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, mosaic scan, lambda scan, FRET macro, FRAP macro | ||
software | |||
incubation | cage incubator | ||
links |
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inv.nr. |
[[Category:{{{type}}}]]