MTZ02 - Leica TCS SP5

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| building            = MTZ
 
| building            = MTZ
 
| room                = B20.001C
 
| room                = B20.001C
| description          = This [[Leica TCS SP5]] [[confocal]] microscope is specialized for Life Cell Imaging, FRET and FRAP. The selected laser equipment makes it suitable for i.e. GFP+mCherry and CFP+YFP. Modules for time-series and bleaching experiments allow to run also more complex experiments, but one can also perform high resolution scans with fixed and embedded samples. The spectral detection system offers flexibility in the combination of dyes. Motorized stage and DIC optics offer the possibility to perform semi-automated microscopy.
+
| description          = This [[Leica TCS SP5]] [[confocal]] microscope is specialized for Life Cell Imaging, FRET and FRAP. Modules for time-series and bleaching experiments are available.
 +
| applications        = fixed cell imaging, life cell imaging, FRET and FRAP, multi position acquisition, [[tile scan]], routine dye combination as well as exotic ones. Optimal for EGFP/mCherry or CFP/YFP/mCherry
 
| microscope          = inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC
 
| microscope          = inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC
 
| objectives          = 10x/0.3<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo
 
| objectives          = 10x/0.3<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo

Revision as of 17:22, 8 July 2008

Suitable for [[applications::fixed cell imaging, life cell imaging, FRET and FRAP, multi position acquisition, tile scan, routine dye combination as well as exotic ones. Optimal for EGFP/mCherry or CFP/YFP/mCherry]].
MTZ02.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MTZ Imaging (MTZ, B20.001C)

Booking

https://cgc.med.tu-dresden.de/phpScheduleIt

Details

microscope , inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC
objectives
illumination
detection point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole
optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel)
reflectors DAPI (EX 360/40, EM LP425)
GFP (EX 470/40, EM LP515)
RFP (EX 538/45, EM LP590)
features [[features::simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, lambda scan, FRET macro, FRAP macro]]
software
incubation cage incubator
links
  • [ manual]
  • [LPM laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr.



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