MTZ02 - Leica TCS SP5
From BioDIP
(Difference between revisions)
m (mtz added) |
(objectives corrected) |
||
Line 12: | Line 12: | ||
| applications = fixed cell imaging, life cell imaging, FRET and FRAP, multi position acquisition, [[tile scan]], routine dye combination as well as exotic ones. Optimal for EGFP/mCherry or CFP/YFP/mCherry | | applications = fixed cell imaging, life cell imaging, FRET and FRAP, multi position acquisition, [[tile scan]], routine dye combination as well as exotic ones. Optimal for EGFP/mCherry or CFP/YFP/mCherry | ||
| microscope = inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC | | microscope = inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC | ||
− | | objectives = 10x/0. | + | | objectives = 10x/0.4 dry<br>20x/0.7 dry<br>40x/1.25 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo |
| illumination = Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 496, 514 nm Argon laser<br>543 nm HeNe laser<br>594 nm HeNe laser<br>633 nm HeNe laser | | illumination = Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 496, 514 nm Argon laser<br>543 nm HeNe laser<br>594 nm HeNe laser<br>633 nm HeNe laser | ||
| detection = point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole<br>optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel) | | detection = point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole<br>optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel) |
Revision as of 11:10, 4 August 2009
Directions
[[image:|150px|text-bottom|right]]
Booking
https://cgc.med.tu-dresden.de/phpScheduleIt
Details
microscope | , inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC | ||
objectives |
| ||
illumination |
| ||
detection | point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel) | ||
reflectors | DAPI (EX 360/40, EM LP425) GFP (EX 470/40, EM LP515) RFP (EX 538/45, EM LP590) | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, lambda scan, FRET macro, FRAP macro | ||
software | |||
incubation | cage incubator | ||
links |
| ||
inv.nr. | - |
[[Category:{{{type}}}]]