MTZ02 - Leica TCS SP5
From BioDIP
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=MTZ02 |
| − | | system-name | + | |system-name=Leica TCS SP5 |
| − | | category | + | |type=Microscope |
| − | | | + | |category=Laser Scanning Confocal |
| − | | | + | |facility=MTZ Imaging |
| − | | | + | |building=MTZ |
| − | | facility_image | + | |room=B20.001C |
| − | + | |facility_image=logo-mtzimaging.jpg | |
| − | + | |description=This [[Leica TCS SP5]] [[confocal]] microscope is specialized for Life Cell Imaging, FRET and FRAP. Modules for time-series and bleaching experiments are available. | |
| − | | description | + | |applications=fixed cell imaging, life cell imaging, FRET and FRAP, multi position acquisition, [[tile scan]], routine dye combination as well as exotic ones. Optimal for EGFP/mCherry or CFP/YFP/mCherry |
| − | | applications | + | |image=MTZ02.jpg |
| − | | microscope | + | |stand=3D |
| − | | objectives | + | |microscope=inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC |
| − | | illumination | + | |objectives=10x/0.4 dry<br>20x/0.7 dry<br>40x/1.25 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo |
| − | | detection | + | |illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 496, 514 nm Argon laser<br>543 nm HeNe laser<br>594 nm HeNe laser<br>633 nm HeNe laser |
| − | | reflectors | + | |detection=point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole<br>optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel) |
| − | | features | + | |reflectors=DAPI (EX 360/40, EM LP425)<br>GFP (EX 470/40, EM LP515)<br>RFP (EX 538/45, EM LP590) |
| − | | incubation | + | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, lambda scan, FRET macro, FRAP macro |
| − | | link1 | + | |incubation=cage incubator (T+CO2) |
| − | | link2 | + | |link1=[http://spreadsheets.google.com/pub?key=pMZJXCgd5HNumJpfihZLcaw LPM] |
| − | | inv | + | |link2=[https://spreadsheets.google.com/pub?key=pMZJXCgd5HNsytK4WLaxaWQ scan-head configuration] |
| + | |inv=- | ||
| + | |obj1=Empty position | ||
| + | |obj2=Empty position | ||
| + | |obj3=Empty position | ||
| + | |obj4=Empty position | ||
| + | |obj5=Empty position | ||
| + | |obj6=Empty position | ||
| + | |obj7=Empty position | ||
| + | |obj8=Empty position | ||
}} | }} | ||
| − | |||
[[Category:Wide-Field|i]] | [[Category:Wide-Field|i]] | ||
Revision as of 16:54, 1 September 2009
|
Directions
[[image:|150px|text-bottom|right]]
Booking
https://cgc.med.tu-dresden.de/phpScheduleIt
Details
| microscope | , 3D stand, inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC | ||
| objectives | |||
| illumination |
| ||
| detection | point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel) | ||
| reflectors | DAPI (EX 360/40, EM LP425) GFP (EX 470/40, EM LP515) RFP (EX 538/45, EM LP590) | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, lambda scan, FRET macro, FRAP macro | ||
| software | |||
| incubation | cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | - |
