MTZ02 - Leica TCS SP5

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{{Equipment
 
{{Equipment
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| system-number       = 02
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|system-number=MTZ02
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| system-name         = Leica TCS SP5
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|system-name=Leica TCS SP5
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| category             = Laser Scanning Confocal
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|type=Microscope
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| stand                = inverted
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|category=Laser Scanning Confocal
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| image                = MTZ02.jpg
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|facility=MTZ Imaging
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| facility             = MTZ Imaging
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|building=MTZ
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| facility_image      = logo-mtzimaging.jpg
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|room=B20.001C
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| building             = MTZ
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|Facility-logo=Logo mtz 3.png
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| room                 = B20.001C
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|Institute-logo=Logo Medizin.png
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| description         = Our Leica TCS SP5 is specialized for Life Cell Imaging, FRET and FRAP. The selected laser equipment makes it suitable for i.e. GFP+mCherry and CFP+YFP. Modules for time-series and bleaching experiments allow to run also more complex experiments, but one can also perform high resolution scans with fixed and embedded samples. The spectral detection system offers flexibility in the combination of dyes. Motorized stage and DIC optics offer the possibility to perform semi-automated microscopy.
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|description=This [[Leica TCS SP5]] [[confocal]] microscope is specialized for Life Cell Imaging, FRET and FRAP. Modules for time-series and bleaching experiments are available.
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| microscope           = inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC
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|applications=fixed cell imaging, life cell imaging, FRET and FRAP, multi position acquisition, [[tile scan]], routine dye combination as well as exotic ones. Optimal for EGFP/mCherry or CFP/YFP/mCherry
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| objectives          = 10x/0.3<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo
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|image=MTZ02.jpg
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| illumination        = Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 496, 514 nm Argon laser<br>543 nm HeNe laser<br>594 nm HeNe laser<br>633 nm HeNe laser
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|stand-name=Leica - DMI6000
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| detection           = point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole
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|stand=Inverted
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| reflectors           = DAPI (EX 360/40, EM LP425)<br>GFP (EX 470/40, EM LP515)<br>RFP (EX 538/45, EM LP590)
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|microscope=inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC
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| features             = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, mosaic scan, lambda scan, FRET macro, FRAP macro
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|obj1=Leica HC PL APO 10x 0.4
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| incubation           = cage incubator
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|obj2=Leica HC PL APO 20x 0.7
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|obj3=Leica HCX PL APO 40x 1.25-0.75 Oil
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|obj4=Leica HCX PL APO 63x 1.4-0.6 Oil
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|obj5=Leica HCX PL APO 63x 1.2 W
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|ill1=Transmitted Light (Halogen)
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|ill2=Fluorescence (HBO)
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|ill3=Laser Diode 405 nm
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|ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm
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|ill5=Laser HeNe 543 nm
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|ill6=Laser HeNe 594 nm
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|ill7=Laser HeNe 633 nm
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|detection=point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole<br>optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel)
 +
|reflectors=DAPI (EX 360/40, EM LP425)<br>GFP (EX 470/40, EM LP515)<br>RFP (EX 538/45, EM LP590)
 +
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, lambda scan, FRET macro, FRAP macro
 +
|incubation=Cage incubator (T)
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|link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNumJpfihZLcaw
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|link2=https://spreadsheets.google.com/pub?key=pMZJXCgd5HNsytK4WLaxaWQ
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|inv=-
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|facility_image=logo-mtzimaging.jpg
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|objectives=10x/0.4 dry<br>20x/0.7 dry<br>40x/1.25 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo
 +
|illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 496, 514 nm Argon laser<br>543 nm HeNe laser<br>594 nm HeNe laser<br>633 nm HeNe laser
 
}}
 
}}
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[[Category:Wide-Field|i]]

Latest revision as of 16:52, 11 November 2013

Suitable for [[applications::fixed cell imaging, life cell imaging, FRET and FRAP, multi position acquisition, tile scan, routine dye combination as well as exotic ones. Optimal for EGFP/mCherry or CFP/YFP/mCherry]].
MTZ02.jpg


[edit] Directions

Logo Medizin.png
Logo mtz 3.pngMTZ Imaging (MTZ, B20.001C)

[edit] Booking

https://cgc.med.tu-dresden.de/phpScheduleIt

[edit] Details

microscope Leica - DMI6000, Inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC
objectives
Leica HC PL APO 10x 0.4
Leica HC PL APO 20x 0.7
Leica HCX PL APO 40x 1.25-0.75 Oil
Leica HCX PL APO 63x 1.4-0.6 Oil
Leica HCX PL APO 63x 1.2 W
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 496, 514 nm
Laser HeNe 543 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
detection point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole
optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel)
reflectors DAPI (EX 360/40, EM LP425)
GFP (EX 470/40, EM LP515)
RFP (EX 538/45, EM LP590)
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, lambda scan, FRET macro, FRAP macro
software
incubation Cage incubator (T)
links
inv.nr. -


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