Papers

Light Microscopy

How to get it right

• Pearson, Helen, 2007: The good, the bad and the ugly
  Nature 447, 138-140 (10 May 2007) source

• Under the microscope
  Nature 447, 116 - 116 (09 May 2007) source

• Pawley, Jim, 2000: The 39 Steps: A Cautionary Tale about “quantatative” 3D Fluorescence Microscopy
  BioTechniques 28-5:2-4 source

Fluorescent Proteins

• Koushik, Srinagesh V. et. al. , 2006: Cerulean, Venus, and VenusY67C FRET Reference Standards
  Biophys J. 2006 December 15; 91(12): L99–L101. source

• Eisenstein, Michael, 2006: Helping cells to tell a colorful tale
  Nature Methods 3, 647 - 655 (01 Aug 2006) source

Techniques

• Donnert, Gerald et al., 2006, Major signal increase in fluorescence microscopy through dark-state relaxation
  Nature Methods 4 (01), 81-86

• Frohn, J. T. et. al., 2001: Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation
  Optics Letters, Vol. 26, Issue 11, pp. 828-830 source

• Göbel, Werner et. al., 2007: Imaging cellular network dynamics in three dimensions using fast 3D laser scanning
  Nature Methods - 4, 73 - 79 (2007) source

• Edward J. Botcherby, Rimas Juskaitis, Martin J. Booth, Tony Wilson, 2007: Aberration-free optical refocusing in high numerical aperture microscopy.
  Opt Lett. 2007 Jul 15;32(14):2007-9 source

Image Processing and Analysis

Colocalization

• Bolte, S. et. al., 2006: A guided tour into subcellular colocalization analysis in light microscopy
  Journal of Microscopy, Vol. 224, Pt 3 December 2006, pp. 213–232 source
  • Good paper to start with if you want to learn more about colocalization. Covers all relevant points.

• Costes, Sylvain V. et. al., 2004: Automatic and Quantitative Measurement of Protein-Protein Colocalization in Live Cells
  Biophysical Journal Volume 86 June 2004 3993–4003 source

• Manders, E.M.M. et. al., 1993: Measurement of co-localization of objects in dual-color confocal images
  Journal of Microscopy, Vol. 169, Pt 3, March 1993, pp. 375-382

• Manders, E.M.M. et. al., 1992: Dynamics of three-dimensional replication patterns during the S-phase, analyzed by double labelling of DNA and confocal microscopy
  Journal of Cell Science 103, 857-862 (1992)

Technical Tests

• Murray, John M. et. al., 2007: Evaluating performance in three-dimensional fluorescence microscopy
  Journal of Microscopy Volume 228 Issue 3 Page 390-405, December 2007 source

• Zucker, Robert M., 2006: Quality Assessment of Confocal Microscopy Slide Based Systems: Performance
  Cytometry Part A 69A:659–676 (2006) source

• Zucker, Robert M., 2006: Quality Assessment of Confocal Microscopy Slide-Based Systems: Instability
  Cytometry Part A 69A:677–690 (2006) source

• Zucker, Robert M., 2007: Reliability of confocal microscopy spectral imaging systems: Use of multispectral beads.
  Cytometry A. 2007 Jan 31 source