PhD Course Nov 09

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|align="center" style="background:Moccasin;"|''Glass cube demo and practise <br> Koehler illumination''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
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| 11:00||12:30||align="center"|LECTURE 1|| align="center" |Basics in Optics <br> Characteristics of light <br> Light sources <br> Reflection, Absorption, Refraction <br>Diffraction-Demo||align="center" |Humberto
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| 10:00||11:00||align="center"|LECTURE 4|| align="center" |The Eye <br> Numerical Aperture <br> Diffraction||align="center" |Humberto
 
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|align="center" style="background:Moccasin;"|''Activity 1 <br> (Group Rotation)''
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|align="center" style="background:Moccasin;"|''Activity 5''
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|align="left" style="background:Moccasin;"|''1) Capillary + oil and Coin in glass <br> 2) Laser refraction + reflection <br> 3) Different immersion objectives - Demo Tirf Obective''||align="left" style="background:Moccasin;"|''1) Pete <br> 2) Silke <br> 3) Britta''
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|align="center" style="background:Moccasin;"|''Diffraction grids / gratings''||align="center" style="background:Moccasin;"|
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|align="center" style="background:Moccasin;"|''Activity 6''
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|align="center" style="background:Moccasin;"|''Airy disk on the microscope on mirror''||align="center" style="background:Moccasin;"|"LMF TEAM"
 
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
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| 13:30||15:30||align="center"|Lecture 2|| align="center" |Introduction to lenses <br> Lens abberration <br> Understanding Ray optics||align="center" |Humberto
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| 11:15||11:30||align="center"|Lecture 5|| align="center" |Aperture (Objective and Condenser) - Cube DEMO||align="center" |Humberto
 
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|align="center" style="background:Moccasin;"|''Activity 2''
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|align="center" style="background:Moccasin;"|''Activity 7''
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|align="center" style="background:Moccasin;"|''Ray optics <br> Drawing ray diagrams''||align="left" style="background:Moccasin;"|
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|align="left" style="background:Moccasin;"|''1) Objective cleaning + NA reading + Coverslip thickness measurement <br> 2) Diffraction Exercise on microscope <br> 3) Fluorescent beads with variable NA''||align="left" style="background:Moccasin;"|1) Pete <br> 2) Dan <br> 3) Britta
 
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|align="center" style="background:Moccasin;"|''Activity 3 <br> (Group Rotation)''
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''
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|align="left" style="background:Moccasin;"|''1) Finding focal point of lenses + infinity + Colour fringes <br> 2) Virtual and real images. 25cm distance <br> 3) Image of Arrow in the microscope (upright and inverted)''||align="left" style="background:Moccasin;"|''1) Jan <br> 2) Humberto <br> 3) Pete + Britta''
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| 14:00||15:45||align="center"|Lecture 6|| align="center" |Contrasting techniques I (Dark field + Phase contrast)||align="center" |Humberto
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|align="center" style="background:Moccasin;"|''Activity 8 <br> (4 groups)''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
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| 15:50||17:00||align="center"|Lecture 3|| align="center" |Microscope components <br> Focusing, Field of View, Depth of field, depth of focus <br> Planes (filament Demo) <br> Conjugated planes <br> Koehler illumination||align="center" |Humberto
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| 14:00||15:45||align="center"|Lecture 7|| align="center" |Contrasting techniques II (Polarization + DIC)||align="center" |Humberto
 
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|align="center" style="background:Moccasin;"|''Activity 4''
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|align="center" style="background:Moccasin;"|''Activity 9 <br> (4 groups)''
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|align="center" style="background:Moccasin;"|''Glass cube demo and practise <br> Koehler illumination''||align="center" style="background:Moccasin;"|''LMF TEAM''
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|align="center" style="background:Moccasin;"|''Polarization foil <br> Polarization on microscope <br> DIC on microsocpe''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
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Revision as of 13:32, 6 November 2009

Contents

PhD Course Nov 09

Preliminary Schedule

Friday 13th Nov

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


DAY ONE (Monday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:30 INTRODUCTION GROUP PRESENTATION
Wishes for the course
The ideal microsocpe		 LMF TEAM
DEMO Optical Bench LMF TEAM
10:30 11:00 BREAK BREAK BREAK
11:00 12:30 LECTURE 1 Basics in Optics
Characteristics of light
Light sources
Reflection, Absorption, Refraction
Diffraction-Demo		 Humberto
Activity 1
(Group Rotation) 		1) Capillary + oil and Coin in glass
2) Laser refraction + reflection
3) Different immersion objectives - Demo Tirf Obective		 1) Pete
2) Silke
3) Britta
12:30 13:30 LUNCH LUNCH LUNCH
13:30 15:30 Lecture 2 Introduction to lenses
Lens abberration
Understanding Ray optics		 Humberto
Activity 2 Ray optics
Drawing ray diagrams
Activity 3
(Group Rotation) 		1) Finding focal point of lenses + infinity + Colour fringes
2) Virtual and real images. 25cm distance
3) Image of Arrow in the microscope (upright and inverted)		 1) Jan
2) Humberto
3) Pete + Britta
15:30 15:50 BREAK BREAK BREAK
15:50 17:00 Lecture 3 Microscope components
Focusing, Field of View, Depth of field, depth of focus
Planes (filament Demo)
Conjugated planes
Koehler illumination		 Humberto
17:00 18:30 Activity 4 Glass cube demo and practise
Koehler illumination		 LMF TEAM


DAY TWO (TUESDAY)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 INTRODUCTION
(groups of 3)		 Koehler Exercise
Questions		 LMF TEAM
10:00 11:00 LECTURE 4 The Eye
Numerical Aperture
Diffraction		 Humberto
Activity 5 Diffraction grids / gratings
Activity 6 Airy disk on the microscope on mirror "LMF TEAM"
11:00 11:15 BREAK BREAK BREAK
11:15 11:30 Lecture 5 Aperture (Objective and Condenser) - Cube DEMO Humberto
11:30 13:00 Activity 7 1) Objective cleaning + NA reading + Coverslip thickness measurement
2) Diffraction Exercise on microscope
3) Fluorescent beads with variable NA		 1) Pete
2) Dan
3) Britta
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:45 Lecture 6 Contrasting techniques I (Dark field + Phase contrast) Humberto
Activity 8
(4 groups) 		Dark field and Phase contrast LMF TEAM
15:45 16:00 BREAK BREAK BREAK
14:00 15:45 Lecture 7 Contrasting techniques II (Polarization + DIC) Humberto
Activity 9
(4 groups) 		Polarization foil
Polarization on microscope
DIC on microsocpe		 LMF TEAM
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