PhD Course Nov 09

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|align="center" style="background:Moccasin;"|''DEMO''
 
|align="center" style="background:Moccasin;"|''DEMO''
 
|align="center" style="background:Moccasin;"|''Optical Bench''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
|align="center" style="background:Moccasin;"|''Optical Bench''||align="center" style="background:Moccasin;"|''LMF TEAM''
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|align="center" style="background:Moccasin;"|''Activity 1 <br> (Group Rotation)''
 
|align="center" style="background:Moccasin;"|''Activity 1 <br> (Group Rotation)''
 
|align="left" style="background:Moccasin;"|''1) Capillary + oil and Coin in glass <br> 2) Laser refraction + reflection <br> 3) Different immersion objectives - Demo Tirf Obective''||align="left" style="background:Moccasin;"|''1) Pete <br> 2) Silke <br> 3) Britta''
 
|align="left" style="background:Moccasin;"|''1) Capillary + oil and Coin in glass <br> 2) Laser refraction + reflection <br> 3) Different immersion objectives - Demo Tirf Obective''||align="left" style="background:Moccasin;"|''1) Pete <br> 2) Silke <br> 3) Britta''
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|align="center" style="background:Moccasin;"|''Activity 2''
 
|align="center" style="background:Moccasin;"|''Activity 2''
 
|align="center" style="background:Moccasin;"|''Ray optics <br> Drawing ray diagrams''||align="left" style="background:Moccasin;"|
 
|align="center" style="background:Moccasin;"|''Ray optics <br> Drawing ray diagrams''||align="left" style="background:Moccasin;"|
 
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|align="center" style="background:Moccasin;"|''Activity 3 <br> (Group Rotation)''
 
|align="center" style="background:Moccasin;"|''Activity 3 <br> (Group Rotation)''
 
|align="left" style="background:Moccasin;"|''1) Finding focal point of lenses + infinity + Colour fringes <br> 2) Virtual and real images. 25cm distance <br> 3) Image of Arrow in the microscope (upright and inverted)''||align="left" style="background:Moccasin;"|''1) Jan <br> 2) Humberto <br> 3) Pete + Britta''
 
|align="left" style="background:Moccasin;"|''1) Finding focal point of lenses + infinity + Colour fringes <br> 2) Virtual and real images. 25cm distance <br> 3) Image of Arrow in the microscope (upright and inverted)''||align="left" style="background:Moccasin;"|''1) Jan <br> 2) Humberto <br> 3) Pete + Britta''
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|align="center" style="background:Moccasin;"|''Glass cube demo and practise <br> Koehler illumination''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
|align="center" style="background:Moccasin;"|''Glass cube demo and practise <br> Koehler illumination''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
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|align="center" style="background:Moccasin;"|''Activity 5''
 
|align="center" style="background:Moccasin;"|''Activity 5''
 
|align="center" style="background:Moccasin;"|''Diffraction grids / gratings''||align="center" style="background:Moccasin;"|
 
|align="center" style="background:Moccasin;"|''Diffraction grids / gratings''||align="center" style="background:Moccasin;"|
 
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|align="center" style="background:Moccasin;"|''Activity 6''
 
|align="center" style="background:Moccasin;"|''Activity 6''
 
|align="center" style="background:Moccasin;"|''Airy disk on the microscope on mirror''||align="center" style="background:Moccasin;"|"LMF TEAM"
 
|align="center" style="background:Moccasin;"|''Airy disk on the microscope on mirror''||align="center" style="background:Moccasin;"|"LMF TEAM"
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|align="center" style="background:Moccasin;"|''Activity 7''
 
|align="center" style="background:Moccasin;"|''Activity 7''
 
|align="left" style="background:Moccasin;"|''1) Objective cleaning + NA reading + Coverslip thickness measurement <br> 2) Diffraction Exercise on microscope <br> 3) Fluorescent beads with variable NA''||align="left" style="background:Moccasin;"|1) Pete <br> 2) Dan <br> 3) Britta
 
|align="left" style="background:Moccasin;"|''1) Objective cleaning + NA reading + Coverslip thickness measurement <br> 2) Diffraction Exercise on microscope <br> 3) Fluorescent beads with variable NA''||align="left" style="background:Moccasin;"|1) Pete <br> 2) Dan <br> 3) Britta
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|align="center" style="background:Moccasin;"|''Activity 8 <br> (4 groups)''
 
|align="center" style="background:Moccasin;"|''Activity 8 <br> (4 groups)''
 
|align="center" style="background:Moccasin;"|''Dark field and Phase contrast''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
|align="center" style="background:Moccasin;"|''Dark field and Phase contrast''||align="center" style="background:Moccasin;"|''LMF TEAM''
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|align="center" style="background:Moccasin;"|''Activity 9 <br> (4 groups)''
 
|align="center" style="background:Moccasin;"|''Activity 9 <br> (4 groups)''
 
|align="center" style="background:Moccasin;"|''Polarization foil <br> Polarization on microscope <br> DIC on microsocpe''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
|align="center" style="background:Moccasin;"|''Polarization foil <br> Polarization on microscope <br> DIC on microsocpe''||align="center" style="background:Moccasin;"|''LMF TEAM''
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=== DAY THREE (Wednesday) ===
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{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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|- style="; color:MidnightBlue;"
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| align="center" style="background:#f0f0f0;"|'''FROM'''
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| align="center" style="background:#f0f0f0;"|'''TO'''
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| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
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| align="center" style="background:#f0f0f0;"|'''TOPICS'''
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| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 
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| 9:00||9:45||align="center"|INTRODUCTION <br> (groups of 3)||align="center" |Questions||align="center" |LMF TEAM
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| 9:45||10:45||align="center"|LECTURE 8|| align="center" |Objectives <br> Objective correction <br> Infinity optics||align="center" |Humberto
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| style="background:DarkKhaki;"|'''10:45'''
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| style="background:DarkKhaki;"|'''11:00'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
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| style="background:Moccasin;"|''11:00''
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| style="background:Moccasin;"|''11:45''
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|align="center" style="background:Moccasin;"|''Activity 10''
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|align="center" style="background:Moccasin;"|''1) Lens Abberration <br> 2) Objective reading <br> 3) Infinity optics on optical bench''||align="center" style="background:Moccasin;"|1) Humberto <br> 2) Silke <br> 3) Jan + Dan
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| 11:45||12:15||align="center"|LECTURE 9|| align="center" |Basics of Fluorescence <br> Fluorochromes||align="center" |Dan
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| style="background:DarkKhaki;"|'''12:15'''
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| style="background:DarkKhaki;"|'''13:15'''
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
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| 13:15||15:00||align="center"|LECTURE 10 (interactive)|| align="center" |Fluorescence Microscopy <br> Filters||align="center" |
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|align="center" style="background:Moccasin;"|''Activity 11''
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|align="center" style="background:Moccasin;"|''Fluorescence imaging''||align="center" style="background:Moccasin;"|"LMF TEAM"
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| 15:00||15:45||align="center"|Lecture 11|| align="center" |CCD||align="center" |Britta
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| style="background:DarkKhaki;"|'''15:45'''
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| style="background:DarkKhaki;"|'''16:00'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
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| 16:00||16:45||align="center"|Lecture 12|| align="center" |Digital Imaging||align="center" |Dan
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| style="background:Moccasin;"|''16:45''
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| style="background:Moccasin;"|''18:30''
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|align="center" style="background:Moccasin;"|''Activity 12''
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|align="center" style="background:Moccasin;"|''Wide field systems + CCD''||align="center" style="background:Moccasin;"|"LMF TEAM"
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=== DAY FOUR (Thursday) ===
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{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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|- style="; color:MidnightBlue;"
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| align="center" style="background:#f0f0f0;"|'''FROM'''
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| align="center" style="background:#f0f0f0;"|'''TO'''
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| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
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| align="center" style="background:#f0f0f0;"|'''TOPICS'''
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| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
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| 9:00||9:45||align="center"|INTRODUCTION <br> (groups of 3)||align="center" |Questions <br> Filter reading||align="center" |LMF TEAM
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| 9:45||10:15||align="center"|LECTURE 13a|| align="center" |Optical sectioning||align="center" |Jan
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| style="background:DarkKhaki;"|'''10:15'''
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| style="background:DarkKhaki;"|'''10:30'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
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| 10:30||12:00||align="center"|LECTURE 13b|| align="center" |Confocal Microscopy <br> Multiphoton microscopy <br> Spinning disc <br> TIRF <br> Project discussions||align="center" |Jan
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| style="background:DarkKhaki;"|'''12:00'''
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| style="background:DarkKhaki;"|'''13:00'''
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
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| style="background:Moccasin;"|''13:00''
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| style="background:Moccasin;"|''14:30''
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|align="center" style="background:Moccasin;"|''Activity 13a''
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|align="center" style="background:Moccasin;"|''SYSTEM ROTATION <br> Leica confocal, Spinning disc, Delta Vision''||align="center" style="background:Moccasin;"|"Pete, Britta, Dan"
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| style="background:DarkKhaki;"|'''14:30'''
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| style="background:DarkKhaki;"|'''14:45'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
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| style="background:Moccasin;"|''14:45''
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| style="background:Moccasin;"|''16:15''
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|align="center" style="background:Moccasin;"|''Activity 13b''
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|align="center" style="background:Moccasin;"|''SYSTEM ROTATION <br> Leica confocal, Spinning disc, Delta Vision''||align="center" style="background:Moccasin;"|"Pete, Britta, Dan"
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| style="background:DarkKhaki;"|'''16:15'''
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| style="background:DarkKhaki;"|'''16:30'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
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| style="background:Moccasin;"|''16:30''
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| style="background:Moccasin;"|''18:00''
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|align="center" style="background:Moccasin;"|''Activity 13c''
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|align="center" style="background:Moccasin;"|''SYSTEM ROTATION <br> Leica confocal, Spinning disc, Delta Vision''||align="center" style="background:Moccasin;"|"Pete, Britta, Dan"
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Revision as of 15:13, 6 November 2009

Contents

PhD Course Nov 09

Preliminary Schedule

Friday 13th Nov

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


DAY ONE (Monday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:30 INTRODUCTION GROUP PRESENTATION
Wishes for the course
The ideal microsocpe		 LMF TEAM
DEMO Optical Bench LMF TEAM
10:30 11:00 BREAK BREAK BREAK
11:00 12:30 LECTURE 1 Basics in Optics
Characteristics of light
Light sources
Reflection, Absorption, Refraction
Diffraction-Demo		 Humberto
Activity 1
(Group Rotation) 		1) Capillary + oil and Coin in glass
2) Laser refraction + reflection
3) Different immersion objectives - Demo Tirf Obective		 1) Pete
2) Silke
3) Britta
12:30 13:30 LUNCH LUNCH LUNCH
13:30 15:30 Lecture 2 Introduction to lenses
Lens abberration
Understanding Ray optics		 Humberto
Activity 2 Ray optics
Drawing ray diagrams
Activity 3
(Group Rotation) 		1) Finding focal point of lenses + infinity + Colour fringes
2) Virtual and real images. 25cm distance
3) Image of Arrow in the microscope (upright and inverted)		 1) Jan
2) Humberto
3) Pete + Britta
15:30 15:50 BREAK BREAK BREAK
15:50 17:00 Lecture 3 Microscope components
Focusing, Field of View, Depth of field, depth of focus
Planes (filament Demo)
Conjugated planes
Koehler illumination		 Humberto
17:00 18:30 Activity 4 Glass cube demo and practise
Koehler illumination		 LMF TEAM


DAY TWO (TUESDAY)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 INTRODUCTION
(groups of 3)		 Koehler Exercise
Questions		 LMF TEAM
10:00 11:00 LECTURE 4 The Eye
Numerical Aperture
Diffraction		 Humberto
Activity 5 Diffraction grids / gratings
Activity 6 Airy disk on the microscope on mirror "LMF TEAM"
11:00 11:15 BREAK BREAK BREAK
11:15 11:30 Lecture 5 Aperture (Objective and Condenser) - Cube DEMO Humberto
Activity 7 1) Objective cleaning + NA reading + Coverslip thickness measurement
2) Diffraction Exercise on microscope
3) Fluorescent beads with variable NA		 1) Pete
2) Dan
3) Britta
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:45 Lecture 6 Contrasting techniques I (Dark field + Phase contrast) Humberto
Activity 8
(4 groups) 		Dark field and Phase contrast LMF TEAM
15:45 16:00 BREAK BREAK BREAK
14:00 15:45 Lecture 7 Contrasting techniques II (Polarization + DIC) Humberto
Activity 9
(4 groups) 		Polarization foil
Polarization on microscope
DIC on microsocpe		 LMF TEAM


DAY THREE (Wednesday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:45 INTRODUCTION
(groups of 3)		 Questions LMF TEAM
9:45 10:45 LECTURE 8 Objectives
Objective correction
Infinity optics		 Humberto
10:45 11:00 BREAK BREAK BREAK
11:00 11:45 Activity 10 1) Lens Abberration
2) Objective reading
3) Infinity optics on optical bench		 1) Humberto
2) Silke
3) Jan + Dan
11:45 12:15 LECTURE 9 Basics of Fluorescence
Fluorochromes		 Dan
12:15 13:15 LUNCH LUNCH LUNCH
13:15 15:00 LECTURE 10 (interactive) Fluorescence Microscopy
Filters
Activity 11 Fluorescence imaging "LMF TEAM"
15:00 15:45 Lecture 11 CCD Britta
15:45 16:00 BREAK BREAK BREAK
16:00 16:45 Lecture 12 Digital Imaging Dan
16:45 18:30 Activity 12 Wide field systems + CCD "LMF TEAM"


DAY FOUR (Thursday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:45 INTRODUCTION
(groups of 3)		 Questions
Filter reading		 LMF TEAM
9:45 10:15 LECTURE 13a Optical sectioning Jan
10:15 10:30 BREAK BREAK BREAK
10:30 12:00 LECTURE 13b Confocal Microscopy
Multiphoton microscopy
Spinning disc
TIRF
Project discussions		 Jan
12:00 13:00 LUNCH LUNCH LUNCH
13:00 14:30 Activity 13a SYSTEM ROTATION
Leica confocal, Spinning disc, Delta Vision		 "Pete, Britta, Dan"
14:30 14:45 BREAK BREAK BREAK
14:45 16:15 Activity 13b SYSTEM ROTATION
Leica confocal, Spinning disc, Delta Vision		 "Pete, Britta, Dan"
16:15 16:30 BREAK BREAK BREAK
16:30 18:00 Activity 13c SYSTEM ROTATION
Leica confocal, Spinning disc, Delta Vision		 "Pete, Britta, Dan"
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