PhD Course Nov 09
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Revision as of 17:48, 11 November 2009
Contents |
PhD Course Nov 09
Friday 13th Nov
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
DAY ONE (Monday)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 10:30 | INTRODUCTION | GROUP PRESENTATION Wishes for the course The ideal microsocpe |
LMF TEAM |
| DEMO | Optical Bench | LMF TEAM | ||
| 10:30 | 11:00 | BREAK | BREAK | BREAK |
| 11:00 | 12:30 | LECTURE 1 | Basics in Optics Characteristics of light Light sources Reflection, Absorption, Refraction Diffraction-Demo |
Humberto |
| Activity 1 (Group Rotation) |
1) Capillary + oil and Coin in glass 2) Laser refraction + reflection 3) Different immersion objectives - Demo Tirf Obective |
1) Pete 2) Silke 3) Britta | ||
| 12:30 | 13:30 | LUNCH | LUNCH | LUNCH |
| 13:30 | 15:30 | Lecture 2 | Introduction to lenses Lens abberration Understanding Ray optics |
Humberto |
| Activity 2 | Ray optics Drawing ray diagrams |
|||
| Activity 3 (Group Rotation) |
1) Finding focal point of lenses + infinity + Colour fringes 2) Virtual and real images. 25cm distance 3) Image of Arrow in the microscope (upright and inverted) |
1) Jan 2) Humberto 3) Pete + Britta | ||
| 15:30 | 15:50 | BREAK | BREAK | BREAK |
| 15:50 | 17:00 | Lecture 3 | Microscope components Focusing, Field of View, Depth of field, depth of focus Planes (filament Demo) Conjugated planes Koehler illumination |
Humberto |
| 17:00 | 18:30 | Activity 4 | Glass cube demo and practise Koehler illumination |
LMF TEAM |
DAY TWO (TUESDAY)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 10:00 | INTRODUCTION (groups of 3) |
Koehler Exercise Questions |
LMF TEAM |
| 10:00 | 11:00 | LECTURE 4 | The Eye Numerical Aperture Diffraction |
Humberto |
| Activity 5 | Diffraction grids / gratings | |||
| Activity 6 | Airy disk on the microscope on mirror | LMF TEAM | ||
| 11:00 | 11:15 | BREAK | BREAK | BREAK |
| 11:15 | 11:30 | DEMO 1 | Aperture (Objective and Condenser) - Cube | Humberto |
| Activity 7 | 1) Objective cleaning + NA reading + Coverslip thickness measurement 2) Diffraction Exercise on microscope 3) Fluorescent beads with variable NA |
1) Pete 2) Dan 3) Britta | ||
| 13:00 | 14:00 | LUNCH | LUNCH | LUNCH |
| 14:00 | 15:45 | Lecture 5 | Contrasting techniques I (Dark field + Phase contrast) | Humberto |
| Activity 8 (4 groups) |
Dark field and Phase contrast | LMF TEAM | ||
| 15:45 | 16:00 | BREAK | BREAK | BREAK |
| 16:00 | 18:00 | Lecture 6 | Contrasting techniques II (Polarization + DIC) | Humberto |
| Activity 9 (4 groups) |
Polarization foil Polarization on microscope DIC on microsocpe |
LMF TEAM |
DAY THREE (Wednesday)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:45 | INTRODUCTION (groups of 3) |
Questions | LMF TEAM |
| 9:45 | 10:45 | LECTURE 7 | Objectives Objective correction Infinity optics |
Humberto |
| 10:45 | 11:00 | BREAK | BREAK | BREAK |
| 11:00 | 11:45 | Activity 10 | 1) Lens Abberration 2) Objective reading 3) Infinity optics on optical bench |
1) Humberto 2) Silke 3) Jan + Dan |
| 11:45 | 12:15 | LECTURE 8 | Basics of Fluorescence Fluorochromes |
Dan |
| 12:15 | 13:15 | LUNCH | LUNCH | LUNCH |
| 13:15 | 15:00 | LECTURE 9 (interactive) | Fluorescence Microscopy Filters |
|
| Activity 11 | Fluorescence imaging | LMF TEAM | ||
| 15:00 | 15:45 | Lecture 10 | CCD | Britta |
| 15:45 | 16:00 | BREAK | BREAK | BREAK |
| 16:00 | 16:45 | Lecture 11 | Digital Imaging | Dan |
| 16:45 | 18:30 | Activity 12 | Mercury lamp exchange and alignment Wide field systems + CCD |
LMF TEAM |
DAY FOUR (Thursday)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:45 | INTRODUCTION (groups of 3) |
Questions Filter reading |
LMF TEAM |
| 9:45 | 10:15 | LECTURE 12a | Optical sectioning | Jan |
| 10:15 | 10:30 | BREAK | BREAK | BREAK |
| 10:30 | 12:00 | LECTURE 12b | Confocal Microscopy Multiphoton microscopy Spinning disc TIRF Project discussions |
Jan |
| 12:00 | 13:00 | LUNCH | LUNCH | LUNCH |
| 13:00 | 14:30 | Activity 13a | SYSTEM ROTATION Leica confocal, Spinning disc, Delta Vision |
Pete, Britta, Dan |
| 14:30 | 14:45 | BREAK | BREAK | BREAK |
| 14:45 | 16:15 | Activity 13b | SYSTEM ROTATION Leica confocal, Spinning disc, Delta Vision |
Pete, Britta, Dan |
| 16:15 | 16:30 | BREAK | BREAK | BREAK |
| 16:30 | 18:00 | Activity 13c | SYSTEM ROTATION Leica confocal, Spinning disc, Delta Vision |
Pete, Britta, Dan |
DAY Five (Friday)
Please bring your own laptop and preinstall Fiji!!!
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 10:00 | INTRODUCTION (groups of 3) |
Questions | LMF TEAM |
| 10:00 | 11:30 | LECTURE 13a | Digital Images What is image processing |
Dan |
| Activity 14 | Fiji (... is just ImageJ) | |||
| 11:30 | 11:45 | BREAK | BREAK | BREAK |
| 11:45 | 13:00 | LECTURE 13b | Image processing | Dan |
| Activity 14 | Fiji (... is just ImageJ) | |||
| 13:00 | 14:00 | LUNCH | LUNCH | LUNCH |
| 14:00 | 15:30 | LMF booking database IFN concept, members and Wiki Final discussion |
IFN |
Office Duty
| FROM | TO | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
| Morning | first break | / | Britta | Dan | Pete | Humberto |
| first break | lunch | Dan | Pete | Britta | Humberto | Silke |
| lunch | break | Silke | Silke | Pete | Humberto Silke |
/ |
| break | end | Pete | Dan | Humberto | Humberto Silke |
/ |
