PhD Course Nov 09
From BioDIP
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| − | | 13:30||15: | + | | 13:30||15:45||align="center"|Lecture 2|| align="center" |Introduction to lenses <br> Lens abberration <br> Understanding Ray optics||align="center" |Humberto |
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| − | | style="background:DarkKhaki;"|'''15: | + | | style="background:DarkKhaki;"|'''15:45''' |
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|align="center" style="background:DarkKhaki;"|'''BREAK''' | |align="center" style="background:DarkKhaki;"|'''BREAK''' | ||
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK''' | |align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK''' | ||
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| − | | | + | | 16:00||17:00||align="center"|Lecture 3|| align="center" |Microscope components <br> Focusing, Field of View, Depth of field, depth of focus <br> Planes (filament Demo) <br> Conjugated planes <br> Koehler illumination||align="center" |Humberto |
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| align="center" style="background:#f0f0f0;width:140px"|'''Responsible person(s)''' | | align="center" style="background:#f0f0f0;width:140px"|'''Responsible person(s)''' | ||
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| 9:00||10:00||align="center"|INTRODUCTION <br> (groups of 3)||align="center" | Koehler Exercise <br> Questions||align="center" |LMF TEAM | | 9:00||10:00||align="center"|INTRODUCTION <br> (groups of 3)||align="center" | Koehler Exercise <br> Questions||align="center" |LMF TEAM | ||
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| − | | 10:00||11: | + | | 10:00||11:25||align="center" style="background:Moccasin;"|''Practical 5'' |
| + | |align="center" style="background:Moccasin;"|''Diffraction grids / gratings''||align="center" style="background:Moccasin;"| | ||
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| − | |align="center | + | |align="center"|LECTURE 4a|| align="center" |Diffraction <br> The Eye <br> Interference||align="center" |Humberto |
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|align="center" style="background:Moccasin;"|''Practical 6'' | |align="center" style="background:Moccasin;"|''Practical 6'' | ||
| − | |align="center" style="background:Moccasin;"|''Airy disk on the microscope | + | |align="center" style="background:Moccasin;"|''Airy disk on the microscope through mirror''||align="center" style="background:Moccasin;"|''LMF TEAM'' |
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| + | |align="center"|LECTURE 4b|| align="center" |Resolution <br> NA||align="center" |Humberto | ||
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| − | | style="background:DarkKhaki;"|'''11: | + | | style="background:DarkKhaki;"|'''11:25''' |
| − | | style="background:DarkKhaki;"|'''11: | + | | style="background:DarkKhaki;"|'''11:40''' |
|align="center" style="background:DarkKhaki;"|'''BREAK''' | |align="center" style="background:DarkKhaki;"|'''BREAK''' | ||
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK''' | |align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK''' | ||
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| − | | style="background:Moccasin;"|''11: | + | | style="background:Moccasin;"|''11:40'' |
| style="background:Moccasin;"|''12:40'' | | style="background:Moccasin;"|''12:40'' | ||
|align="center" style="background:Moccasin;"|''Practical 7'' | |align="center" style="background:Moccasin;"|''Practical 7'' | ||
| − | |align="left" style="background:Moccasin;"|''1 | + | |align="left" style="background:Moccasin;"|''1) NA Objectives - angle measurements <br> 2) Diffraction Exercise on microscope <br> 3) Fluorescent beads with variable NA''||align="left" style="background:Moccasin;"|1) Silke <br> 2) Dan <br> 3) Britta |
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Revision as of 16:08, 19 November 2009
Contents |
PhD Course Nov 09
Friday 13th Nov
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
DAY ONE (Monday)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 10:30 | INTRODUCTION | GROUP PRESENTATION Wishes for the course The ideal microsocpe |
LMF TEAM |
| DEMO | Optical Bench | LMF TEAM | ||
| 10:30 | 11:00 | BREAK | BREAK | BREAK |
| 11:00 | 12:30 | LECTURE 1 | Basics in Optics Characteristics of light Light sources Reflection, Absorption, Refraction Diffraction-Demo |
Humberto |
| Practical 1 (Group Rotation) |
1) Capillary + oil and Coin in glass 2) Laser refraction + reflection 3) Different immersion objectives - Demo Tirf Obective + DV oil box |
1) Pete 2) Silke 3) Britta | ||
| 12:30 | 13:30 | LUNCH | LUNCH | LUNCH |
| 13:30 | 15:45 | Lecture 2 | Introduction to lenses Lens abberration Understanding Ray optics |
Humberto |
| Practical 2 | Ray optics Drawing ray diagrams |
|||
| Practical 3 (Group Rotation) |
1) Finding focal point of lenses + infinity + Colour fringes 2) Virtual and real images. 25cm distance 3) Image of Arrow in the microscope (upright and inverted) |
1) Jan 2) Humberto 3) Pete + Britta | ||
| 15:45 | 16:00 | BREAK | BREAK | BREAK |
| 16:00 | 17:00 | Lecture 3 | Microscope components Focusing, Field of View, Depth of field, depth of focus Planes (filament Demo) Conjugated planes Koehler illumination |
Humberto |
| 17:00 | 18:30 | Practical 4 | Glass cube demo and practise Focusing; Depth of field / focus Koehler illumination |
LMF TEAM |
DAY TWO (TUESDAY)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 10:00 | INTRODUCTION (groups of 3) |
Koehler Exercise Questions |
LMF TEAM |
| 10:00 | 11:25 | Practical 5 | Diffraction grids / gratings | |
| LECTURE 4a | Diffraction The Eye Interference |
Humberto | ||
| Practical 6 | Airy disk on the microscope through mirror | LMF TEAM | ||
| LECTURE 4b | Resolution NA |
Humberto | ||
| 11:25 | 11:40 | BREAK | BREAK | BREAK |
| 11:40 | 12:40 | Practical 7 | 1) NA Objectives - angle measurements 2) Diffraction Exercise on microscope 3) Fluorescent beads with variable NA |
1) Silke 2) Dan 3) Britta |
| 12:40 | 13:40 | LUNCH | LUNCH | LUNCH |
| 13:40 | 15:30 | Lecture 5 | Contrasting techniques I Dark field + Phase contrast |
Humberto |
| Practical 8 (4 groups) |
Dark field and Phase contrast | LMF TEAM | ||
| 15:30 | 15:45 | BREAK | BREAK | BREAK |
| 15:45 | 17:45 | Lecture 6 | Contrasting techniques II Polarization + DIC |
Humberto |
| Practical 9 (4 groups) |
Polarization foil Polarization on microscope DIC on microsocpe |
LMF TEAM |
DAY THREE (Wednesday)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:45 | INTRODUCTION (groups of 3) |
Questions | LMF TEAM |
| 9:45 | 10:45 | LECTURE 7 | Objectives Objective correction Infinity optics |
Humberto |
| 10:45 | 11:00 | BREAK | BREAK | BREAK |
| 11:00 | 11:45 | Practical 10 | 1) Lens Abberration 2) Objective reading and cleaning, coverslip thickness measurement 3) Infinity optics on optical bench |
1) Humberto 2) Silke 3) Jan |
| 11:45 | 12:15 | LECTURE 8 | Basics of Fluorescence Fluorochromes |
Dan |
| 12:15 | 13:15 | LUNCH | LUNCH | LUNCH |
| 13:15 | 15:00 | LECTURE 9 (interactive) | Fluorescence Microscopy Filters |
Silke |
| Practical 11 | Fluorescence imaging | LMF TEAM | ||
| 15:00 | 15:45 | Lecture 10 | CCD | Britta |
| 15:45 | 16:00 | BREAK | BREAK | BREAK |
| 16:00 | 16:45 | Lecture 11 | Digital Imaging | Dan |
| 16:45 | 18:30 | Practical 12 (4 groups) | Mercury lamp exchange and alignment Wide field systems + CCD |
LMF TEAM |
Checklist for Widefield/CCD practical
- Exposure Time
- Gain
- Binning
- ROI
- Saturation
- Histogram
- Gamma
- Mapping/LUTs
- Images - Display
- Scale - ruler
- Pixel size
DAY FOUR (Thursday)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:45 | INTRODUCTION (groups of 3) |
Questions Filter reading |
LMF TEAM |
| 9:45 | 10:15 | LECTURE 12a | Optical sectioning | Dan |
| 10:15 | 10:30 | BREAK | BREAK | BREAK |
| 10:30 | 12:00 | LECTURE 12b | Confocal Microscopy Multiphoton microscopy Spinning disc TIRF Project discussions |
Dan |
| 12:00 | 13:00 | LUNCH | LUNCH | LUNCH |
| 13:00 | 14:30 | Practical 13a | SYSTEM ROTATION Leica confocal, Spinning disc, Delta Vision |
Pete, Britta, Dan |
| 14:30 | 14:45 | BREAK | BREAK | BREAK |
| 14:45 | 16:15 | Practical 13b | SYSTEM ROTATION Leica confocal, Spinning disc, Delta Vision |
Pete, Britta, Dan |
| 16:15 | 16:30 | BREAK | BREAK | BREAK |
| 16:30 | 18:00 | Practical 13c | SYSTEM ROTATION Leica confocal, Spinning disc, Delta Vision |
Pete, Britta, Dan |
DAY Five (Friday)
Please bring your own laptop and preinstall Fiji!!!
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 10:00 | INTRODUCTION (groups of 3) |
Questions | LMF TEAM |
| 10:00 | 11:30 | LECTURE 13a | Digital Images What is image processing |
Dan |
| Practical 14 | Fiji (... is just ImageJ) | |||
| 11:30 | 11:45 | BREAK | BREAK | BREAK |
| 11:45 | 13:00 | LECTURE 13b | Image processing | Dan |
| Practical 14 | Fiji (... is just ImageJ) | |||
| 13:00 | 14:00 | LUNCH | LUNCH | LUNCH |
| 14:00 | 15:30 | LMF booking database IFN concept, members and Wiki Final discussion |
IFN | |
| 15:30 | 16:00 | Evaluation | LMF TEAM |
Office Duty
| FROM | TO | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
| Morning | first break | / | Britta | Dan | Pete | Britta |
| first break | lunch | Dan | Pete | Britta | Humberto | Silke |
| lunch | break | Silke | Silke | Pete | Humberto Silke |
/ |
| break | end | Pete | Dan | Humberto | Humberto Silke |
/ |
