PhD Course Nov 09

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==PhD Course Nov 09 ==
 
==PhD Course Nov 09 ==
  
−
== Preliminary Schedule ==
 
 
=== Friday 13th Nov ===
 
=== Friday 13th Nov ===
 
Setup - all lmf staff move stuff to galleria
 
Setup - all lmf staff move stuff to galleria
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{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 
|- style="; color:MidnightBlue;"
 
|- style="; color:MidnightBlue;"
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| align="center" style="background:#f0f0f0;"|'''FROM'''
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| align="center" style="background:#f0f0f0;width:40px"|'''FROM'''
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| align="center" style="background:#f0f0f0;"|'''TO'''
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| align="center" style="background:#f0f0f0;width:40px"|'''TO'''
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| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
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| align="center" style="background:#f0f0f0;width:140px"|'''ACTIVITY'''
−
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
+
| align="center" style="background:#f0f0f0;width:400px"|'''TOPICS'''
−
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
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| align="center" style="background:#f0f0f0;width:140px"|'''Responsible person(s)'''
 
|-
 
|-
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| ||||||||
 
 
|-
 
|-
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| 9:30||10:30||align="center"|INTRODUCTION||align="center" | GROUP PRESENTATION <br> Wishes for the course <br> The ideal microsocpe||align="center" |LMF TEAM
+
| 9:00||10:30||align="center"|INTRODUCTION||align="center" | GROUP PRESENTATION <br> Wishes for the course <br> The ideal microsocpe||align="center" |LMF TEAM
 
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| style="background:#f0f0f0;"|
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||
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|align="center" style="background:Moccasin;"|''DEMO''
 
|align="center" style="background:Moccasin;"|''DEMO''
 
|align="center" style="background:Moccasin;"|''Optical Bench''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
|align="center" style="background:Moccasin;"|''Optical Bench''||align="center" style="background:Moccasin;"|''LMF TEAM''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
 
| 11:00||12:30||align="center"|LECTURE 1|| align="center" |Basics in Optics <br> Characteristics of light <br> Light sources <br> Reflection, Absorption, Refraction <br>Diffraction-Demo||align="center" |Humberto
 
| 11:00||12:30||align="center"|LECTURE 1|| align="center" |Basics in Optics <br> Characteristics of light <br> Light sources <br> Reflection, Absorption, Refraction <br>Diffraction-Demo||align="center" |Humberto
 
|-
 
|-
 
|-
 
|-
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| style="background:#f0f0f0;"|''' '''
+
||
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| style="background:#f0f0f0;"|''' '''
+
||
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|align="center" style="background:Moccasin;"|''Activity 1 <br> (Group Rotation)''
+
|align="center" style="background:Moccasin;"|''Practical 1 <br> (Group Rotation)''
−
|align="left" style="background:Moccasin;"|''1) Capillary + oil and Coin in glass <br> 2) Laser refraction + reflection <br> 3) Different immersion objectives - Demo Tirf Obective''||align="left" style="background:Moccasin;"|''1) Pete <br> 2) Silke <br> 3) Britta''
+
|align="left" style="background:Moccasin;"|''1) Capillary + oil and Coin in glass <br> 2) Laser refraction + reflection <br> 3) Different immersion objectives - Demo Tirf Obective + DV oil box''||align="left" style="background:Moccasin;"|''1) Pete <br> 2) Silke <br> 3) Britta''
−
|-
+
−
| ||||||||
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|-
 
|-
 
|-
 
|-
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
−
| 13:30||15:30||align="center"|Lecture 2|| align="center" |Introduction to lenses <br> Lens abberration <br> Understanding Ray optics||align="center" |Humberto
+
| 13:30||15:45||align="center"|Lecture 2|| align="center" |Introduction to lenses <br> Lens abberration <br> Understanding Ray optics||align="center" |Humberto
 
|-
 
|-
 
|-
 
|-
−
| style="background:#f0f0f0;"|''' '''
+
||
−
| style="background:#f0f0f0;"|''' '''
+
||
−
|align="center" style="background:Moccasin;"|''Activity 2''
+
|align="center" style="background:Moccasin;"|''Practical 2''
 
|align="center" style="background:Moccasin;"|''Ray optics <br> Drawing ray diagrams''||align="left" style="background:Moccasin;"|
 
|align="center" style="background:Moccasin;"|''Ray optics <br> Drawing ray diagrams''||align="left" style="background:Moccasin;"|
 
|-
 
|-
 
|-
 
|-
−
| style="background:#f0f0f0;"|''' '''
+
||
−
| style="background:#f0f0f0;"|''' '''
+
||
−
|align="center" style="background:Moccasin;"|''Activity 3 <br> (Group Rotation)''
+
|align="center" style="background:Moccasin;"|''Practical 3 <br> (Group Rotation)''
 
|align="left" style="background:Moccasin;"|''1) Finding focal point of lenses + infinity + Colour fringes <br> 2) Virtual and real images. 25cm distance <br> 3) Image of Arrow in the microscope (upright and inverted)''||align="left" style="background:Moccasin;"|''1) Jan <br> 2) Humberto <br> 3) Pete + Britta''
 
|align="left" style="background:Moccasin;"|''1) Finding focal point of lenses + infinity + Colour fringes <br> 2) Virtual and real images. 25cm distance <br> 3) Image of Arrow in the microscope (upright and inverted)''||align="left" style="background:Moccasin;"|''1) Jan <br> 2) Humberto <br> 3) Pete + Britta''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
−
| style="background:DarkKhaki;"|'''15:30'''
+
| style="background:DarkKhaki;"|'''15:45'''
−
| style="background:DarkKhaki;"|'''15:50'''
+
| style="background:DarkKhaki;"|'''16:00'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
−
| 15:50||17:00||align="center"|Lecture 3|| align="center" |Microscope components <br> Focusing, Field of View, Depth of field, depth of focus <br> Planes (filament Demo) <br> Conjugated planes <br> Koehler illumination||align="center" |Humberto
+
| 16:00||17:00||align="center"|Lecture 3|| align="center" |Microscope components <br> Focusing, Field of View, Depth of field, depth of focus <br> Planes (filament Demo) <br> Conjugated planes <br> Koehler illumination||align="center" |Humberto
 
|-
 
|-
 
|-
 
|-
 
| style="background:Moccasin;"|''17:00''
 
| style="background:Moccasin;"|''17:00''
 
| style="background:Moccasin;"|''18:30''
 
| style="background:Moccasin;"|''18:30''
−
|align="center" style="background:Moccasin;"|''Activity 4''
+
|align="center" style="background:Moccasin;"|''Practical 4''
−
|align="center" style="background:Moccasin;"|''Glass cube demo and practise <br> Koehler illumination''||align="center" style="background:Moccasin;"|''LMF TEAM''
+
|align="center" style="background:Moccasin;"|''Glass cube demo and practise <br> Focusing; Depth of field / focus<br> Koehler illumination''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
|-
 
|-
−
| ||||||||
 
 
|}
 
|}
  
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{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 
|- style="; color:MidnightBlue;"
 
|- style="; color:MidnightBlue;"
−
| align="center" style="background:#f0f0f0;"|'''FROM'''
+
| align="center" style="background:#f0f0f0;width:40px"|'''FROM'''
−
| align="center" style="background:#f0f0f0;"|'''TO'''
+
| align="center" style="background:#f0f0f0;width:40px"|'''TO'''
−
| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
+
| align="center" style="background:#f0f0f0;width:140px"|'''ACTIVITY'''
−
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
+
| align="center" style="background:#f0f0f0;width:400px"|'''TOPICS'''
−
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
+
| align="center" style="background:#f0f0f0;width:140px"|'''Responsible person(s)'''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
 
| 9:00||10:00||align="center"|INTRODUCTION <br> (groups of 3)||align="center" | Koehler Exercise <br> Questions||align="center" |LMF TEAM
 
| 9:00||10:00||align="center"|INTRODUCTION <br> (groups of 3)||align="center" | Koehler Exercise <br> Questions||align="center" |LMF TEAM
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
−
| 10:00||11:00||align="center"|LECTURE 4|| align="center" |The Eye <br> Numerical Aperture <br> Diffraction||align="center" |Humberto
+
| 10:00||11:25||align="center" style="background:Moccasin;"|''Practical 5''
 +
|align="center" style="background:Moccasin;"|''Diffraction grids / gratings''||align="center" style="background:Moccasin;"|
 
|-
 
|-
 
|-
 
|-
−
| style="background:#f0f0f0;"|''' '''
+
||
−
| style="background:#f0f0f0;"|''' '''
+
||
−
|align="center" style="background:Moccasin;"|''Activity 5''
+
|align="center"|LECTURE 4a|| align="center" |Diffraction <br> The Eye <br> Interference||align="center" |Humberto
−
|align="center" style="background:Moccasin;"|''Diffraction grids / gratings''||align="center" style="background:Moccasin;"|
+
 
|-
 
|-
 
|-
 
|-
−
| style="background:#f0f0f0;"|''' '''
+
||
−
| style="background:#f0f0f0;"|''' '''
+
||
−
|align="center" style="background:Moccasin;"|''Activity 6''
+
|align="center" style="background:Moccasin;"|''Practical 6''
−
|align="center" style="background:Moccasin;"|''Airy disk on the microscope on mirror''||align="center" style="background:Moccasin;"|"LMF TEAM"
+
|align="center" style="background:Moccasin;"|''Airy disk on the microscope through mirror''||align="center" style="background:Moccasin;"|''LMF TEAM''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
 +
||
 +
||
 +
|align="center"|LECTURE 4b|| align="center" |Resolution <br> NA||align="center" |Humberto
 
|-
 
|-
−
| style="background:DarkKhaki;"|'''11:00'''
+
|-
−
| style="background:DarkKhaki;"|'''11:15'''
+
| style="background:DarkKhaki;"|'''11:25'''
 +
| style="background:DarkKhaki;"|'''11:40'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
−
| 11:15||11:30||align="center"|Lecture 5|| align="center" |Aperture (Objective and Condenser) - Cube DEMO||align="center" |Humberto
+
| style="background:Moccasin;"|''11:40''
 +
| style="background:Moccasin;"|''12:40''
 +
|align="center" style="background:Moccasin;"|''Practical 7''
 +
|align="left" style="background:Moccasin;"|''1) NA Objectives - angle measurements <br> 2) Diffraction Exercise on microscope <br> 3) Fluorescent beads with variable NA''||align="left" style="background:Moccasin;"|1) Silke <br> 2) Dan <br> 3) Britta
 
|-
 
|-
 
|-
 
|-
−
| style="background:#f0f0f0;"|''11:30''
+
| style="background:DarkKhaki;"|'''12:40'''
−
| style="background:#f0f0f0;"|''13:00''
+
| style="background:DarkKhaki;"|'''13:40'''
−
|align="center" style="background:Moccasin;"|''Activity 7''
+
|align="center" style="background:DarkKhaki;"|'''LUNCH'''
−
|align="left" style="background:Moccasin;"|''1) Objective cleaning + NA reading + Coverslip thickness measurement <br> 2) Diffraction Exercise on microscope <br> 3) Fluorescent beads with variable NA''||align="left" style="background:Moccasin;"|1) Pete <br> 2) Dan <br> 3) Britta
+
|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
−
| style="background:DarkKhaki;"|'''13:00'''
+
| 13:40||15:30||align="center"|Lecture 5a|| align="center" |Contrasting techniques I <br> Dark field ||align="center" |Humberto
−
| style="background:DarkKhaki;"|'''14:00'''
+
|-
 +
|-
 +
||
 +
||
 +
|align="center" style="background:Moccasin;"|''Practical 8a <br> (4 groups)''
 +
|align="center" style="background:Moccasin;"|''Dark field''||align="center" style="background:Moccasin;"|''LMF TEAM''
 +
|-
 +
|-
 +
||
 +
||
 +
|align="center"|Lecture 5b|| align="center" |Contrasting techniques I <br> Phase contrast||align="center" |Humberto
 +
|-
 +
|-
 +
||
 +
||
 +
|align="center" style="background:Moccasin;"|''Practical 8b <br> (4 groups)''
 +
|align="center" style="background:Moccasin;"|''Phase contrast''||align="center" style="background:Moccasin;"|''LMF TEAM''
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''15:30'''
 +
| style="background:DarkKhaki;"|'''15:45'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
|-
 +
| 15:45||17:45||align="center"|Lecture 6a|| align="center" |Contrasting techniques II <br> Polarization||align="center" |Humberto
 +
|-
 +
|-
 +
||
 +
||
 +
|align="center" style="background:Moccasin;"|''Practical 9a <br> (4 groups)''
 +
|align="center" style="background:Moccasin;"|''Polarization''||align="center" style="background:Moccasin;"|''LMF TEAM''
 +
|-
 +
|-
 +
||
 +
||
 +
|align="center"|Lecture 6b|| align="center" |Contrasting techniques II <br>Differential Interference Contrast||align="center" |Humberto
 +
|-
 +
|-
 +
||
 +
||
 +
|align="center" style="background:Moccasin;"|''Practical 9b <br> (4 groups)''
 +
|align="center" style="background:Moccasin;"|''DIC''||align="center" style="background:Moccasin;"|''LMF TEAM''
 +
|-
 +
|}
 +
 
 +
 
 +
=== DAY THREE (Wednesday) ===
 +
 
 +
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 +
|- style="; color:MidnightBlue;"
 +
| align="center" style="background:#f0f0f0;width:40px"|'''FROM'''
 +
| align="center" style="background:#f0f0f0;width:40px"|'''TO'''
 +
| align="center" style="background:#f0f0f0;width:140px"|'''ACTIVITY'''
 +
| align="center" style="background:#f0f0f0;width:400px"|'''TOPICS'''
 +
| align="center" style="background:#f0f0f0;width:140px"|'''Responsible person(s)'''
 +
|-
 +
|-
 +
| 9:00||9:45||align="center"|INTRODUCTION <br> (groups of 3)||align="center" |Questions||align="center" |LMF TEAM
 +
|-
 +
|-
 +
| 9:45||10:30||align="center"|LECTURE 7|| align="center" |Objectives <br> Objective correction <br> Infinity optics||align="center" |Humberto
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''10:30'''
 +
| style="background:DarkKhaki;"|'''10:45'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
|-
 +
| style="background:Moccasin;"|''10:45''
 +
| style="background:Moccasin;"|''11:45''
 +
|align="center" style="background:Moccasin;"|''Practical 10''
 +
|align="center" style="background:Moccasin;"|''1) Lens Abberration <br> 2) Objective reading and cleaning <br> 3) Infinity optics on optical bench, coverslip thickness measurement''||align="center" style="background:Moccasin;"|1) Humberto <br> 2) Silke <br> 3) Jan
 +
|-
 +
|-
 +
| 11:45||12:30||align="center"|LECTURE 8|| align="center" |Basics of Fluorescence <br> Fluorochromes||align="center" |Dan
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''12:30'''
 +
| style="background:DarkKhaki;"|'''13:30'''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 
|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
−
| 14:00||15:45||align="center"|Lecture 6|| align="center" |Contrasting techniques I (Dark field + Phase contrast)||align="center" |Humberto
+
| 13:30||15:00||align="center"|LECTURE 9 (interactive)|| align="center" |Fluorescence Microscopy <br> Filters||align="center" |Silke
 
|-
 
|-
 
|-
 
|-
−
| style="background:Moccasin;"|
+
||
−
| style="background:Moccasin;"|
+
||
−
|align="center" style="background:Moccasin;"|''Activity 8 <br> (4 groups)''
+
|align="center" style="background:Moccasin;"|''Practical 11''
−
|align="center" style="background:Moccasin;"|''Dark field and Phase contrast''||align="center" style="background:Moccasin;"|''LMF TEAM''
+
|align="center" style="background:Moccasin;"|''Mercury lamp exchange and alignment <br> Fluorescence imaging''||align="center" style="background:Moccasin;"|''LMF TEAM''
 +
|-
 +
|-
 +
| 15:00||15:45||align="center"|Lecture 10|| align="center" |CCD||align="center" |Britta
 +
|-
 
|-
 
|-
 
|-
 
|-
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 
|-
 
|-
−
| ||||||||
 
 
|-
 
|-
−
| 14:00||15:45||align="center"|Lecture 7|| align="center" |Contrasting techniques II (Polarization + DIC)||align="center" |Humberto
+
| 16:00||16:45||align="center"|Lecture 11|| align="center" |Digital Imaging||align="center" |Dan
 
|-
 
|-
 
|-
 
|-
−
| style="background:Moccasin;"|
+
| style="background:Moccasin;"|''16:45''
−
| style="background:Moccasin;"|
+
| style="background:Moccasin;"|''18:30''
−
|align="center" style="background:Moccasin;"|''Activity 9 <br> (4 groups)''
+
|align="center" style="background:Moccasin;"|''Practical 12 (4 groups)''
−
|align="center" style="background:Moccasin;"|''Polarization foil <br> Polarization on microscope <br> DIC on microsocpe''||align="center" style="background:Moccasin;"|''LMF TEAM''
+
|align="center" style="background:Moccasin;"|''Wide field systems + CCD''||align="center" style="background:Moccasin;"|''LMF TEAM''
 +
|-
 +
|}
 +
 
 +
 
 +
==== Checklist for Widefield/CCD practical ====
 +
 
 +
* Exposure Time
 +
** Gain
 +
** Binning
 +
** ROI
 +
** Saturation
 +
** Histogram
 +
** Gamma<br>
 +
* Mapping/LUTs <br>
 +
* Images - Display <br>
 +
* Scale - ruler <br>
 +
* Pixel size
 +
 
 +
 
 +
 
 +
=== DAY FOUR (Thursday) ===
 +
 
 +
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 +
|- style="; color:MidnightBlue;"
 +
| align="center" style="background:#f0f0f0;width:40px"|'''FROM'''
 +
| align="center" style="background:#f0f0f0;width:40px"|'''TO'''
 +
| align="center" style="background:#f0f0f0;width:140px"|'''ACTIVITY'''
 +
| align="center" style="background:#f0f0f0;width:400px"|'''TOPICS'''
 +
| align="center" style="background:#f0f0f0;width:140px"|'''Responsible person(s)'''
 +
|-
 +
|-
 +
| 9:00||9:30||align="center"|INTRODUCTION <br> (groups of 3)||align="center" |Questions <br> Filter reading||align="center" |LMF TEAM
 +
|-
 +
|-
 +
| 9:30||09:45||||align="center"|DEMO - CCD - Colour||align="center" |Britta
 +
|-
 +
|-
 +
| 9:45||10:15||align="center"|LECTURE 12a|| align="center" |Optical sectioning||align="center" |Dan
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''10:15'''
 +
| style="background:DarkKhaki;"|'''10:30'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
|-
 +
| 10:30||11:45||align="center"|LECTURE 12b|| align="center" |Confocal Microscopy <br> Multiphoton microscopy <br> Spinning disc <br> TIRF||align="center" |Dan
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''11:45'''
 +
| style="background:DarkKhaki;"|'''12:45'''
 +
|align="center" style="background:DarkKhaki;"|'''LUNCH'''
 +
|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 +
|-
 +
|-
 +
| style="background:Moccasin;"|''12:45''
 +
| style="background:Moccasin;"|''14:15''
 +
|align="center" style="background:Moccasin;"|''Practical 13a''
 +
|align="center" style="background:Moccasin;"|''SYSTEM ROTATION <br> Leica confocal, Spinning disc, Delta Vision''||align="center" style="background:Moccasin;"|''Pete, Britta, Dan''
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''14:15'''
 +
| style="background:DarkKhaki;"|'''14:30'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
|-
 +
| style="background:Moccasin;"|''14:30''
 +
| style="background:Moccasin;"|''16:00''
 +
|align="center" style="background:Moccasin;"|''Practical 13b''
 +
|align="center" style="background:Moccasin;"|''SYSTEM ROTATION <br> Leica confocal, Spinning disc, Delta Vision''||align="center" style="background:Moccasin;"|''Pete, Britta, Dan''
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''16:00'''
 +
| style="background:DarkKhaki;"|'''<font color = red> 17:00 / <font color = black> 16:15'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''<font color = red> SEMINAR / <font color = black> BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
|-
 +
| style="background:Moccasin;"|''<font color = red>17:00 / <font color = black>16:15''
 +
| style="background:Moccasin;"|''<font color = red>18:30 / <font color = black>17:45''
 +
|align="center" style="background:Moccasin;"|''Practical 13c''
 +
|align="center" style="background:Moccasin;"|''SYSTEM ROTATION <br> Leica confocal, Spinning disc, Delta Vision''||align="center" style="background:Moccasin;"|''Pete, Britta, Dan''
 +
|-
 +
|}
 +
 
 +
 
 +
=== DAY Five (Friday) ===
 +
 
 +
''Please bring your own laptop and preinstall Fiji!!!''
 +
 
 +
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 +
|- style="; color:MidnightBlue;"
 +
| align="center" style="background:#f0f0f0;width:40px"|'''FROM'''
 +
| align="center" style="background:#f0f0f0;width:40px"|'''TO'''
 +
| align="center" style="background:#f0f0f0;width:140px"|'''ACTIVITY'''
 +
| align="center" style="background:#f0f0f0;width:400px"|'''TOPICS'''
 +
| align="center" style="background:#f0f0f0;width:140px"|'''Responsible person(s)'''
 
|-
 
|-
 
| ||||||||
 
| ||||||||
 +
|-
 +
| 9:00||10:00||align="center"|INTRODUCTION <br> (groups of 3)||align="center" |Questions||align="center" |LMF TEAM
 +
|-
 +
|-
 +
| 10:00||11:30||align="center"|LECTURE 13a|| align="center" |Digital Images <br> What is image processing||align="center" |Dan
 +
|-
 +
|-
 +
||
 +
||
 +
|align="center" style="background:Moccasin;"|''Practical 14''
 +
|align="center" style="background:Moccasin;"|''Fiji (... is just ImageJ)''||align="center" style="background:Moccasin;"|
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''11:30'''
 +
| style="background:DarkKhaki;"|'''11:45'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 +
|-
 +
|-
 +
| 11:45||13:00||align="center"|LECTURE 13b|| align="center" |Image processing||align="center" |Dan
 +
|-
 +
|-
 +
||
 +
||
 +
|align="center" style="background:Moccasin;"|''Practical 14''
 +
|align="center" style="background:Moccasin;"|''Fiji (... is just ImageJ)''||align="center" style="background:Moccasin;"|
 +
|-
 +
|-
 +
| style="background:DarkKhaki;"|'''13:00'''
 +
| style="background:DarkKhaki;"|'''14:00'''
 +
|align="center" style="background:DarkKhaki;"|'''LUNCH'''
 +
|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 +
|-
 +
|-
 +
| 14:00||15:30|||| align="center" |LMF booking database <br> IFN concept, members and Wiki <br> Final discussion ||align="center" |IFN
 +
|-
 +
|-
 +
| 15:30||16:00|||| align="center" |Evaluation ||align="center" |LMF TEAM
 +
|-
 +
|}
 +
 +
 +
 +
=== Office Duty ===
 +
 +
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
 +
|- style="; color:MidnightBlue;"
 +
| align="center" style="background:#f0f0f0;width:55px"|'''FROM'''
 +
| align="center" style="background:#f0f0f0;width:55px"|'''TO'''
 +
| align="center" style="background:Darkseagreen;width:125px"|'''MONDAY'''
 +
| align="center" style="background:Darkseagreen;width:125px"|'''TUESDAY'''
 +
| align="center" style="background:Darkseagreen;width:125px"|'''WEDNESDAY'''
 +
| align="center" style="background:Darkseagreen;width:125px"|'''THURSDAY'''
 +
| align="center" style="background:Darkseagreen;width:125px"|'''FRIDAY'''
 +
|-
 +
| align="center" style="background:Darkseagreen"|Morning
 +
| align="center" style="background:Darkseagreen"|first break
 +
| align="center"|/||align="center" | Britta||align="center" |Dan||align="center" |Pete||align="center" |Britta
 +
|-
 +
| align="center" style="background:Darkseagreen"|first break
 +
| align="center" style="background:Darkseagreen"|lunch
 +
| align="center"|Dan||align="center" | Pete||align="center" |Britta||align="center" |Humberto||align="center" |Silke
 +
|-
 +
| align="center" style="background:Darkseagreen"|lunch
 +
| align="center" style="background:Darkseagreen"|break
 +
| align="center"|Silke||align="center" |Silke||align="center" |Pete||align="center" |Humberto <br> Silke||align="center" |/
 +
|-
 +
| align="center" style="background:Darkseagreen"|break
 +
| align="center" style="background:Darkseagreen"|end
 +
| align="center"|Pete||align="center" | Dan||align="center" |Humberto||align="center" |Humberto <br> Silke||align="center" |/
 +
|-
 
|}
 
|}

Latest revision as of 16:57, 19 November 2009

Contents

[edit] PhD Course Nov 09

[edit] Friday 13th Nov

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


[edit] DAY ONE (Monday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:30 INTRODUCTION GROUP PRESENTATION
Wishes for the course
The ideal microsocpe		 LMF TEAM
DEMO Optical Bench LMF TEAM
10:30 11:00 BREAK BREAK BREAK
11:00 12:30 LECTURE 1 Basics in Optics
Characteristics of light
Light sources
Reflection, Absorption, Refraction
Diffraction-Demo		 Humberto
Practical 1
(Group Rotation) 		1) Capillary + oil and Coin in glass
2) Laser refraction + reflection
3) Different immersion objectives - Demo Tirf Obective + DV oil box		 1) Pete
2) Silke
3) Britta
12:30 13:30 LUNCH LUNCH LUNCH
13:30 15:45 Lecture 2 Introduction to lenses
Lens abberration
Understanding Ray optics		 Humberto
Practical 2 Ray optics
Drawing ray diagrams
Practical 3
(Group Rotation) 		1) Finding focal point of lenses + infinity + Colour fringes
2) Virtual and real images. 25cm distance
3) Image of Arrow in the microscope (upright and inverted)		 1) Jan
2) Humberto
3) Pete + Britta
15:45 16:00 BREAK BREAK BREAK
16:00 17:00 Lecture 3 Microscope components
Focusing, Field of View, Depth of field, depth of focus
Planes (filament Demo)
Conjugated planes
Koehler illumination		 Humberto
17:00 18:30 Practical 4 Glass cube demo and practise
Focusing; Depth of field / focus
Koehler illumination		 LMF TEAM


[edit] DAY TWO (TUESDAY)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 INTRODUCTION
(groups of 3)		 Koehler Exercise
Questions		 LMF TEAM
10:00 11:25 Practical 5 Diffraction grids / gratings
LECTURE 4a Diffraction
The Eye
Interference		 Humberto
Practical 6 Airy disk on the microscope through mirror LMF TEAM
LECTURE 4b Resolution
NA		 Humberto
11:25 11:40 BREAK BREAK BREAK
11:40 12:40 Practical 7 1) NA Objectives - angle measurements
2) Diffraction Exercise on microscope
3) Fluorescent beads with variable NA		 1) Silke
2) Dan
3) Britta
12:40 13:40 LUNCH LUNCH LUNCH
13:40 15:30 Lecture 5a Contrasting techniques I
Dark field 		Humberto
Practical 8a
(4 groups) 		Dark field LMF TEAM
Lecture 5b Contrasting techniques I
Phase contrast		 Humberto
Practical 8b
(4 groups) 		Phase contrast LMF TEAM
15:30 15:45 BREAK BREAK BREAK
15:45 17:45 Lecture 6a Contrasting techniques II
Polarization		 Humberto
Practical 9a
(4 groups) 		Polarization LMF TEAM
Lecture 6b Contrasting techniques II
Differential Interference Contrast		 Humberto
Practical 9b
(4 groups) 		DIC LMF TEAM


[edit] DAY THREE (Wednesday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:45 INTRODUCTION
(groups of 3)		 Questions LMF TEAM
9:45 10:30 LECTURE 7 Objectives
Objective correction
Infinity optics		 Humberto
10:30 10:45 BREAK BREAK BREAK
10:45 11:45 Practical 10 1) Lens Abberration
2) Objective reading and cleaning
3) Infinity optics on optical bench, coverslip thickness measurement		 1) Humberto
2) Silke
3) Jan
11:45 12:30 LECTURE 8 Basics of Fluorescence
Fluorochromes		 Dan
12:30 13:30 LUNCH LUNCH LUNCH
13:30 15:00 LECTURE 9 (interactive) Fluorescence Microscopy
Filters		 Silke
Practical 11 Mercury lamp exchange and alignment
Fluorescence imaging		 LMF TEAM
15:00 15:45 Lecture 10 CCD Britta
15:45 16:00 BREAK BREAK BREAK
16:00 16:45 Lecture 11 Digital Imaging Dan
16:45 18:30 Practical 12 (4 groups) Wide field systems + CCD LMF TEAM


[edit] Checklist for Widefield/CCD practical

  • Exposure Time
    • Gain
    • Binning
    • ROI
    • Saturation
    • Histogram
    • Gamma
  • Mapping/LUTs
  • Images - Display
  • Scale - ruler
  • Pixel size


[edit] DAY FOUR (Thursday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 INTRODUCTION
(groups of 3)		 Questions
Filter reading		 LMF TEAM
9:30 09:45 DEMO - CCD - Colour Britta
9:45 10:15 LECTURE 12a Optical sectioning Dan
10:15 10:30 BREAK BREAK BREAK
10:30 11:45 LECTURE 12b Confocal Microscopy
Multiphoton microscopy
Spinning disc
TIRF		 Dan
11:45 12:45 LUNCH LUNCH LUNCH
12:45 14:15 Practical 13a SYSTEM ROTATION
Leica confocal, Spinning disc, Delta Vision		 Pete, Britta, Dan
14:15 14:30 BREAK BREAK BREAK
14:30 16:00 Practical 13b SYSTEM ROTATION
Leica confocal, Spinning disc, Delta Vision		 Pete, Britta, Dan
16:00 17:00 / 16:15 BREAK SEMINAR / BREAK BREAK
17:00 / 16:15 18:30 / 17:45 Practical 13c SYSTEM ROTATION
Leica confocal, Spinning disc, Delta Vision		 Pete, Britta, Dan


[edit] DAY Five (Friday)

Please bring your own laptop and preinstall Fiji!!!

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 INTRODUCTION
(groups of 3)		 Questions LMF TEAM
10:00 11:30 LECTURE 13a Digital Images
What is image processing		 Dan
Practical 14 Fiji (... is just ImageJ)
11:30 11:45 BREAK BREAK BREAK
11:45 13:00 LECTURE 13b Image processing Dan
Practical 14 Fiji (... is just ImageJ)
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:30 LMF booking database
IFN concept, members and Wiki
Final discussion 		IFN
15:30 16:00 Evaluation LMF TEAM


[edit] Office Duty

FROM TO MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY
Morning first break / Britta Dan Pete Britta
first break lunch Dan Pete Britta Humberto Silke
lunch break Silke Silke Pete Humberto
Silke		 /
break end Pete Dan Humberto Humberto
Silke		 /

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