PhD Course Nov 09

Latest revision as of 16:57, 19 November 2009

Setup - all lmf staff move stuff to galleria Equipment needed

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	10:30	INTRODUCTION	GROUP PRESENTATION Wishes for the course The ideal microsocpe	LMF TEAM
		DEMO	Optical Bench	LMF TEAM
10:30	11:00	BREAK	BREAK	BREAK
11:00	12:30	LECTURE 1	Basics in Optics Characteristics of light Light sources Reflection, Absorption, Refraction Diffraction-Demo	Humberto
		Practical 1 (Group Rotation)	1) Capillary + oil and Coin in glass 2) Laser refraction + reflection 3) Different immersion objectives - Demo Tirf Obective + DV oil box	1) Pete 2) Silke 3) Britta
12:30	13:30	LUNCH	LUNCH	LUNCH
13:30	15:45	Lecture 2	Introduction to lenses Lens abberration Understanding Ray optics	Humberto
		Practical 2	Ray optics Drawing ray diagrams
		Practical 3 (Group Rotation)	1) Finding focal point of lenses + infinity + Colour fringes 2) Virtual and real images. 25cm distance 3) Image of Arrow in the microscope (upright and inverted)	1) Jan 2) Humberto 3) Pete + Britta
15:45	16:00	BREAK	BREAK	BREAK
16:00	17:00	Lecture 3	Microscope components Focusing, Field of View, Depth of field, depth of focus Planes (filament Demo) Conjugated planes Koehler illumination	Humberto
17:00	18:30	Practical 4	Glass cube demo and practise Focusing; Depth of field / focus Koehler illumination	LMF TEAM

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	10:00	INTRODUCTION (groups of 3)	Koehler Exercise Questions	LMF TEAM
10:00	11:25	Practical 5	Diffraction grids / gratings
		LECTURE 4a	Diffraction The Eye Interference	Humberto
		Practical 6	Airy disk on the microscope through mirror	LMF TEAM
		LECTURE 4b	Resolution NA	Humberto
11:25	11:40	BREAK	BREAK	BREAK
11:40	12:40	Practical 7	1) NA Objectives - angle measurements 2) Diffraction Exercise on microscope 3) Fluorescent beads with variable NA	1) Silke 2) Dan 3) Britta
12:40	13:40	LUNCH	LUNCH	LUNCH
13:40	15:30	Lecture 5a	Contrasting techniques I Dark field	Humberto
		Practical 8a (4 groups)	Dark field	LMF TEAM
		Lecture 5b	Contrasting techniques I Phase contrast	Humberto
		Practical 8b (4 groups)	Phase contrast	LMF TEAM
15:30	15:45	BREAK	BREAK	BREAK
15:45	17:45	Lecture 6a	Contrasting techniques II Polarization	Humberto
		Practical 9a (4 groups)	Polarization	LMF TEAM
		Lecture 6b	Contrasting techniques II Differential Interference Contrast	Humberto
		Practical 9b (4 groups)	DIC	LMF TEAM

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	9:45	INTRODUCTION (groups of 3)	Questions	LMF TEAM
9:45	10:30	LECTURE 7	Objectives Objective correction Infinity optics	Humberto
10:30	10:45	BREAK	BREAK	BREAK
10:45	11:45	Practical 10	1) Lens Abberration 2) Objective reading and cleaning 3) Infinity optics on optical bench, coverslip thickness measurement	1) Humberto 2) Silke 3) Jan
11:45	12:30	LECTURE 8	Basics of Fluorescence Fluorochromes	Dan
12:30	13:30	LUNCH	LUNCH	LUNCH
13:30	15:00	LECTURE 9 (interactive)	Fluorescence Microscopy Filters	Silke
		Practical 11	Mercury lamp exchange and alignment Fluorescence imaging	LMF TEAM
15:00	15:45	Lecture 10	CCD	Britta
15:45	16:00	BREAK	BREAK	BREAK
16:00	16:45	Lecture 11	Digital Imaging	Dan
16:45	18:30	Practical 12 (4 groups)	Wide field systems + CCD	LMF TEAM

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	9:30	INTRODUCTION (groups of 3)	Questions Filter reading	LMF TEAM
9:30	09:45		DEMO - CCD - Colour	Britta
9:45	10:15	LECTURE 12a	Optical sectioning	Dan
10:15	10:30	BREAK	BREAK	BREAK
10:30	11:45	LECTURE 12b	Confocal Microscopy Multiphoton microscopy Spinning disc TIRF	Dan
11:45	12:45	LUNCH	LUNCH	LUNCH
12:45	14:15	Practical 13a	SYSTEM ROTATION Leica confocal, Spinning disc, Delta Vision	Pete, Britta, Dan
14:15	14:30	BREAK	BREAK	BREAK
14:30	16:00	Practical 13b	SYSTEM ROTATION Leica confocal, Spinning disc, Delta Vision	Pete, Britta, Dan
16:00	17:00 / 16:15	BREAK	SEMINAR / BREAK	BREAK
17:00 / 16:15	18:30 / 17:45	Practical 13c	SYSTEM ROTATION Leica confocal, Spinning disc, Delta Vision	Pete, Britta, Dan

Please bring your own laptop and preinstall Fiji!!!

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)

9:00	10:00	INTRODUCTION (groups of 3)	Questions	LMF TEAM
10:00	11:30	LECTURE 13a	Digital Images What is image processing	Dan
		Practical 14	Fiji (... is just ImageJ)
11:30	11:45	BREAK	BREAK	BREAK
11:45	13:00	LECTURE 13b	Image processing	Dan
		Practical 14	Fiji (... is just ImageJ)
13:00	14:00	LUNCH	LUNCH	LUNCH
14:00	15:30		LMF booking database IFN concept, members and Wiki Final discussion	IFN
15:30	16:00		Evaluation	LMF TEAM

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+|align="center" style="background:DarkKhaki;"|'''<font color = red> SEMINAR / <font color = black> BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
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 |align="center" style="background:Moccasin;"|''Practical 13c''
 |align="center" style="background:Moccasin;"|''SYSTEM ROTATION <br> Leica confocal, Spinning disc, Delta Vision''||align="center" style="background:Moccasin;"|''Pete, Britta, Dan''