PhD Course Nov 09
From BioDIP
Contents |
PhD Course Nov 09
Preliminary Schedule
Friday 13th Nov
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
DAY ONE (Monday)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:30 | 10:30 | INTRODUCTION | GROUP PRESENTATION Wishes for the course The ideal microsocpe |
LMF TEAM |
| DEMO | Optical Bench | LMF TEAM | ||
| 10:30 | 11:00 | BREAK | BREAK | BREAK |
| 11:00 | 12:30 | LECTURE 1 | Basics in Optics Characteristics of light Light sources Reflection, Absorption, Refraction Diffraction-Demo |
Humberto |
| Activity 1 (Group Rotation) |
1) Capillary + oil and Coin in glass 2) Laser refraction + reflection 3) Different immersion objectives - Demo Tirf Obective |
1) Pete 2) Silke 3) Britta | ||
| 12:30 | 13:30 | LUNCH | LUNCH | LUNCH |
| 13:30 | 15:30 | Lecture 2 | Introduction to lenses Lens abberration Understanding Ray optics |
Humberto |
| Activity 2 | Ray optics Drawing ray diagrams |
|||
| Activity 3 (Group Rotation) |
1) Finding focal point of lenses + infinity + Colour fringes 2) Virtual and real images. 25cm distance 3) Image of Arrow in the microscope (upright and inverted) |
1) Jan 2) Humberto 3) Pete + Britta | ||
| 15:30 | 15:50 | BREAK | BREAK | BREAK |
| 15:50 | 17:00 | Lecture 3 | Microscope components Focusing, Field of View, Depth of field, depth of focus Planes (filament Demo) Conjugated planes Koehler illumination |
Humberto |
| 17:00 | 18:30 | Activity 4 | Glass cube demo and practise Koehler illumination |
LMF TEAM |
DAY TWO (TUESDAY)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 10:00 | INTRODUCTION (groups of 3) |
Koehler Exercise Questions |
LMF TEAM |
| 10:00 | 11:00 | LECTURE 4 | The Eye Numerical Aperture Diffraction |
Humberto |
| Activity 5 | Diffraction grids / gratings | |||
| Activity 6 | Airy disk on the microscope on mirror | "LMF TEAM" | ||
| 11:00 | 11:15 | BREAK | BREAK | BREAK |
| 11:15 | 11:30 | Lecture 5 | Aperture (Objective and Condenser) - Cube DEMO | Humberto |
| 11:30 | 13:00 | Activity 7 | 1) Objective cleaning + NA reading + Coverslip thickness measurement 2) Diffraction Exercise on microscope 3) Fluorescent beads with variable NA |
1) Pete 2) Dan 3) Britta |
| 13:00 | 14:00 | LUNCH | LUNCH | LUNCH |
| 14:00 | 15:45 | Lecture 6 | Contrasting techniques I (Dark field + Phase contrast) | Humberto |
| Activity 8 (4 groups) |
Dark field and Phase contrast | LMF TEAM | ||
| 15:45 | 16:00 | BREAK | BREAK | BREAK |
| 14:00 | 15:45 | Lecture 7 | Contrasting techniques II (Polarization + DIC) | Humberto |
| Activity 9 (4 groups) |
Polarization foil Polarization on microscope DIC on microsocpe |
LMF TEAM | ||
