SD1 - Andor Spinning Disc
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{{Equipment | {{Equipment | ||
− | |system-number=MPI-SD1 | + | |system-number=MPI- SD1 |
|system-name=Andor Spinning Disc | |system-name=Andor Spinning Disc | ||
|type=Microscope | |type=Microscope | ||
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|building=MPI-CBG | |building=MPI-CBG | ||
|room=038B | |room=038B | ||
− | |Facility-logo=MPI-CBG | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
+ | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=Fast, "real-time" confocal imaging. Laser equipment: 488 nm solid-state Coherent Sapphire (75mW), 561 nm solid-state Cobolt Jive (75mW) + 640 Coherent Cube (40mW) . | |description=Fast, "real-time" confocal imaging. Laser equipment: 488 nm solid-state Coherent Sapphire (75mW), 561 nm solid-state Cobolt Jive (75mW) + 640 Coherent Cube (40mW) . | ||
|applications=confocal imaging of EGFP, mCherry, dTomato, Draq5 and the like with high temporal and spatial resolution. | |applications=confocal imaging of EGFP, mCherry, dTomato, Draq5 and the like with high temporal and spatial resolution. | ||
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|stand=Inverted | |stand=Inverted | ||
|microscope=manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage | |microscope=manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage | ||
− | |obj1=Olympus | + | |obj1=Olympus UPlanSApochromat 10x 0.4 |
|obj2=Olympus UPlanFluar 20x 0.5 | |obj2=Olympus UPlanFluar 20x 0.5 | ||
− | |obj3=Olympus | + | |obj3=Olympus UPlanFluarN 60x 0.9 |
− | |obj4=Olympus | + | |obj4=Olympus UPlanSApochromat 60x 1.20 W |
− | |obj5=Olympus | + | |obj5=Olympus UPlanSApochromat 100x 1.4 Oil |
− | |obj6=Olympus | + | |obj6=Olympus UPlanSApochromat 40x 0.9 |
+ | |obj7=Olympus UApochromat 40x 1.15 W | ||
+ | |obj8=Olympus UPlanFLN 40x 0.75 Ph2 | ||
+ | |obj9=Olympus UPlanFluar 60x 1.25 Oil Ph3 | ||
+ | |obj10=Olympus UApochromatN340 40x 1.15 W | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (HBO) | |ill2=Fluorescence (HBO) | ||
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for use with Neo: with 10x air objective: 0.692um/px (1x optovar); 0.432um/px (1.6x opt.); with 40x air obj.: 0.17um/px (1x opt.), 0.106um/px (1.6x opt.); with 60x W obj.: 0.114um/px (1x opt.), 0.071um/px (1.6x opt.); with 100x oil obj.: 0.068um/px (1x opt.), 0.043um/px (1.6x opt.)<br> | for use with Neo: with 10x air objective: 0.692um/px (1x optovar); 0.432um/px (1.6x opt.); with 40x air obj.: 0.17um/px (1x opt.), 0.106um/px (1.6x opt.); with 60x W obj.: 0.114um/px (1x opt.), 0.071um/px (1.6x opt.); with 100x oil obj.: 0.068um/px (1x opt.), 0.043um/px (1.6x opt.)<br> | ||
<br>old measure pixel sizes before major upgrade 26th - 30th March 2012:<br>image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um<br>camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes | <br>old measure pixel sizes before major upgrade 26th - 30th March 2012:<br>image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um<br>camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes | ||
− | |reflectors=Optical Insight DualView image splitter (currently not installed): <br>holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70<br>holder for GFP/RFP: HC 520/35; BS 565; HC 628/40<br> | + | |reflectors=Optical Insight DualView image splitter (currently not installed): <br>holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70<br>holder for GFP/RFP: HC 520/35; BS 565; HC 628/40<br>Rotr filter wheel: <br>position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP<br> microscope filter turret:<br> position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer |
− | |features=micro point with 15Hz cutter laser with 365nm and 405nm dye cells | + | |features=micro point with 15Hz cutter laser with 365nm and 405nm dye cells; |
− | |software=iQ 2. | + | objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database! |
+ | |software=iQ 2.9 + iQ 3.0 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide] | ||
|incubation=Stage incubator (T) + objective heater | |incubation=Stage incubator (T) + objective heater | ||
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD1_-_Olympus_Andor_Spinning_Disc_-_startup_%26_shutdown | |link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD1_-_Olympus_Andor_Spinning_Disc_-_startup_%26_shutdown | ||
− | |link1= | + | |link1=https://docs.google.com/spreadsheets/d/e/2PACX-1vTk8Go8RU6zjlkxbDYnk-G3zZHYaBoWH0E0R3fsNVspWuo67nw0JqbTYMnhDw47N_u4kIbARjkhMN7t/pubhtml |
|link2=- | |link2=- | ||
|inv=0 10.1.65.208 | |inv=0 10.1.65.208 | ||
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}} | }} |
Latest revision as of 12:41, 26 January 2018
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
microscope | Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage | ||
objectives |
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illumination | |||
detection | [[detection::Spinning disc scan head Yokogawa CSU-X1 (10.000rpm): dichromatic mirrors: Quad band T-405/488/568/647 Triple band T-405/488/561 Single band T-488 physical pinhole radius: 25um, physical pinhole spacing: 250um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor Dual Camera Port TuCam with two cameras and 0.95x projecting lens for each camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um | ||
reflectors | Optical Insight DualView image splitter (currently not installed): holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70 holder for GFP/RFP: HC 520/35; BS 565; HC 628/40 Rotr filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer | ||
features | micro point with 15Hz cutter laser with 365nm and 405nm dye cells;
objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!"micro point with 15Hz cutter laser with 365nm and 405nm dye cells; objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!" cannot be used as a page name in this wiki. | ||
software | [[software::iQ 2.9 + iQ 3.0 (both with Python) iQ3 user guide]] | ||
incubation | Stage incubator (T) + objective heater | ||
links |
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inv.nr. | 0 10.1.65.208 |