SD1 - Andor Spinning Disc
From BioDIP
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=MPI19 |
| − | | system-name | + | |system-name=Andor Spinning Disc |
| − | | category | + | |type=Microscope |
| − | + | |category=Real-Time Confocal | |
| − | + | |facility=MPI-CBG LMF | |
| − | | facility | + | |building=MPI-CBG |
| − | + | |room=038B | |
| − | | building | + | |facility_image=logo-lmf.jpg |
| − | | room | + | |description=fast, "real-time" confocal imaging |
| − | | description | + | |applications=confocal imaging of EGFP and mCherry with high temporal and spatial resolution. |
| − | | applications | + | |image=MPI19.jpg |
| − | | microscope | + | |stand=Inverted |
| − | | objectives | + | |microscope=manual Olympus IX71, 1.6x OptoVar, fast piezo z-drive for objective |
| − | | illumination | + | |objectives=10x / 0.30 Plan-Neofluar<br>60x / 1.20 W UPlanSApo<br>100x / 1.40 Oil UPlanSApo |
| − | | detection | + | |illumination=Halogen<br>HBO<br>488 nm solid-state laser Coherent Sapphire, 75mW<br>561 nm solid-state laser Cobolt Jive, 40mW |
| − | | reflectors | + | |detection=Spinning disc scan head Yokogawa CSU10: dichromatic mirror: [http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=855&CategoryID=82 T-405/488/568/647]<br> physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um |
| − | + | |reflectors=Optical Insight DualView image splitter: <br>HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: blocked, 8: blocked, 9: 716/40<br> microscope filter turret:<br> position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | |
| − | | incubation | + | |incubation=stage incubator (T) + objective heater |
| − | | link1 | + | |link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSkKjMo69HOPSg |
| − | | link2 | + | |link2=- |
| − | | | + | |obj1=- |
| + | |obj2=- | ||
| + | |obj3=- | ||
| + | |obj4=- | ||
| + | |obj5=- | ||
| + | |obj6=- | ||
| + | |obj7=- | ||
| + | |obj8=- | ||
}} | }} | ||
Revision as of 17:38, 1 September 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , Inverted stand, manual Olympus IX71, 1.6x OptoVar, fast piezo z-drive for objective | ||
| objectives | |||
| illumination |
| ||
| detection | [[detection::Spinning disc scan head Yokogawa CSU10: dichromatic mirror: T-405/488/568/647 physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um]] | ||
| reflectors | Optical Insight DualView image splitter: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: blocked, 8: blocked, 9: 716/40 microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | ||
| features | |||
| software | |||
| incubation | stage incubator (T) + objective heater | ||
| links |
| ||
| inv.nr. |
