SD1 - Andor Spinning Disc
From BioDIP
(Difference between revisions)
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|room=038B | |room=038B | ||
|facility_image=logo-lmf.jpg | |facility_image=logo-lmf.jpg | ||
− | |description= | + | |description=Fast, "real-time" confocal imaging. Laser equipment: 488 nm solid-state Coherent Sapphire (75mW) + 561 nm solid-state Cobolt Jive (40mW). |
|applications=confocal imaging of EGFP and mCherry with high temporal and spatial resolution. | |applications=confocal imaging of EGFP and mCherry with high temporal and spatial resolution. | ||
|image=MPI19.jpg | |image=MPI19.jpg | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=manual Olympus IX71, 1.6x OptoVar, fast piezo z-drive for objective | |microscope=manual Olympus IX71, 1.6x OptoVar, fast piezo z-drive for objective | ||
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|obj1=Zeiss EC Plan-Neofluar 10x 0.3 | |obj1=Zeiss EC Plan-Neofluar 10x 0.3 | ||
|obj2=- | |obj2=- | ||
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|obj7=- | |obj7=- | ||
|obj8=- | |obj8=- | ||
+ | |ill1=Transmitted Light (Halogen) | ||
+ | |ill2=Fluorescence (HBO) | ||
+ | |ill3=Laser DPSS 488 nm | ||
+ | |ill4=Laser DPSS 561 nm | ||
+ | |ill5=- | ||
+ | |ill6=- | ||
+ | |ill7=- | ||
+ | |ill8=- | ||
+ | |detection=Spinning disc scan head Yokogawa CSU10: dichromatic mirror: [http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=855&CategoryID=82 T-405/488/568/647]<br> physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um | ||
+ | |reflectors=Optical Insight DualView image splitter: <br>HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: blocked, 8: blocked, 9: 716/40<br> microscope filter turret:<br> position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | ||
+ | |features=- | ||
+ | |incubation=stage incubator (T) + objective heater | ||
+ | |link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSkKjMo69HOPSg | ||
+ | |link2=- | ||
+ | |inv=0 | ||
+ | |objectives=10x / 0.30 Plan-Neofluar<br>60x / 1.20 W UPlanSApo<br>100x / 1.40 Oil UPlanSApo | ||
+ | |illumination=Halogen<br>HBO<br>488 nm solid-state laser Coherent Sapphire, 75mW<br>561 nm solid-state laser Cobolt Jive, 40mW | ||
}} | }} |
Revision as of 15:57, 7 September 2009
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | , Inverted stand, manual Olympus IX71, 1.6x OptoVar, fast piezo z-drive for objective | ||
objectives | |||
illumination | |||
detection | [[detection::Spinning disc scan head Yokogawa CSU10: dichromatic mirror: T-405/488/568/647 physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um]] | ||
reflectors | Optical Insight DualView image splitter: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: blocked, 8: blocked, 9: 716/40 microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | ||
features | - | ||
software | |||
incubation | stage incubator (T) + objective heater | ||
links |
| ||
inv.nr. | 0 |