SD1 - Andor Spinning Disc

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(exchanged Zeiss 10x Objecive by proper Olympus one (UPlanSApo 10x 0.4))
Line 13: Line 13:
 
|stand=Inverted
 
|stand=Inverted
 
|microscope=manual Olympus IX71, 1.6x OptoVar, fast piezo z-drive for objective
 
|microscope=manual Olympus IX71, 1.6x OptoVar, fast piezo z-drive for objective
|obj1=Zeiss EC Plan-Neofluar 10x 0.3
+
|obj1=Olympus UPlanSApo 10x 0.4
 
|obj2=Olympus UPlanSApo 60x 1.20 W
 
|obj2=Olympus UPlanSApo 60x 1.20 W
 
|obj3=Olympus UPlanSApo 100x 1.4 Oil
 
|obj3=Olympus UPlanSApo 100x 1.4 Oil

Revision as of 16:37, 15 February 2010

Suitable for confocal imaging of EGFP and mCherry with high temporal and spatial resolution..
MPI19.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope , Inverted stand, manual Olympus IX71, 1.6x OptoVar, fast piezo z-drive for objective
objectives
Olympus UPlanSApo 10x 0.4
Olympus UPlanSApo 60x 1.20 W
Olympus UPlanSApo 100x 1.4 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
Laser DPSS 488 nm
Laser DPSS 561 nm
detection [[detection::Spinning disc scan head Yokogawa CSU10: dichromatic mirror: T-405/488/568/647
physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)
Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um]]
reflectors Optical Insight DualView image splitter:
HC 520/35; BS 565; HC 628/40
Sutter filter wheel:
position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: blocked, 8: blocked, 9: 716/40
microscope filter turret:
position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer
features -
software iQ 1.9.1
incubation Stage incubator (T) + objective heater
links
  • [- beam path]
  • [ advanced system check]
inv.nr. 0



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