SD1 - Andor Spinning Disc
From BioDIP
(Difference between revisions)
(update fo pixel sizes after repair, cleaning and re-alignment of IX 71 scope) |
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|stand-name=Olympus - IX71 | |stand-name=Olympus - IX71 | ||
|stand=Inverted | |stand=Inverted | ||
| − | |microscope=manual, 1.6x OptoVar, fast piezo z- | + | |microscope=manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage |
|obj1=Olympus UPlanSApo 10x 0.4 | |obj1=Olympus UPlanSApo 10x 0.4 | ||
|obj2=Olympus UPlanSApo 60x 1.20 W | |obj2=Olympus UPlanSApo 60x 1.20 W | ||
Revision as of 12:36, 24 March 2011
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage | ||
| objectives | |||
| illumination | |||
| detection | [[detection::Spinning disc scan head Yokogawa CSU10: dichromatic mirror: T-405/488/568/647 physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes]] | ||
| reflectors | Optical Insight DualView image splitter: holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70 holder for GFP/RFP: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: 716/40 microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | ||
| features | - | ||
| software | iQ 1.9.1 | ||
| incubation | Stage incubator (T) + objective heater | ||
| links |
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| inv.nr. | 0 |
