SD1 - Andor Spinning Disc

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(edit after major upgrade: scan head, objectives, iQ version)
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|building=MPI-CBG
 
|building=MPI-CBG
 
|room=038B
 
|room=038B
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|description=Fast, "real-time" confocal imaging. Laser equipment: 488 nm solid-state Coherent Sapphire (75mW) + 561 nm solid-state Cobolt Jive (75mW).
+
|Facility-logo=MPI-CBG LMF.jpg
−
|applications=confocal imaging of EGFP and mCherry with high temporal and spatial resolution.
+
|description=Fast, "real-time" confocal imaging. Laser equipment: 488 nm solid-state Coherent Sapphire (75mW), 561 nm solid-state Cobolt Jive (75mW) + 640 Coherent Cube (40mW) .
 +
|applications=confocal imaging of EGFP, mCherry, dTomato, Draq5 and the like with high temporal and spatial resolution.
 
|image=MPI19.jpg
 
|image=MPI19.jpg
 
|stand-name=Olympus - IX71
 
|stand-name=Olympus - IX71
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|microscope=manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage
 
|microscope=manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage
 
|obj1=Olympus UPlanSApo 10x 0.4
 
|obj1=Olympus UPlanSApo 10x 0.4
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|obj2=Olympus UPlanSApo 60x 1.20 W
+
|obj2=Olympus UPlanApo 20x 0.7
−
|obj3=Olympus UPlanSApo 100x 1.4 Oil
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|obj3=Olympus UPlan FluarN 60x 0.9
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|obj4=Olympus UPlan FluarN 60x 0.9
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|obj4=Olympus UPlanSApo 60x 1.20 W
 +
|obj5=Olympus UPlanSApo 100x 1.4 Oil
 
|ill1=Transmitted Light (Halogen)
 
|ill1=Transmitted Light (Halogen)
 
|ill2=Fluorescence (HBO)
 
|ill2=Fluorescence (HBO)
 
|ill3=Laser DPSS 488 nm
 
|ill3=Laser DPSS 488 nm
 
|ill4=Laser DPSS 561 nm
 
|ill4=Laser DPSS 561 nm
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|detection=Spinning disc scan head Yokogawa CSU10: dichromatic mirror: [http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=855&CategoryID=82 T-405/488/568/647]<br> physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um<br>camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes
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|ill5=Laser DPSS 640 nm
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|reflectors=Optical Insight DualView image splitter: <br>holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70<br>holder for GFP/RFP: HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40,  1: open,  2: 525/30,  3: razor edge 568LP,  4: dual emitter: 512/23+630/91,  5: razor edge 488LP,  6: old CSU dual emitter:  525/30 + 650/?,  7: 605/70,  8: 445/40,  9: 716/40<br> microscope filter turret:<br> position 1: GFP cube,  2: RFP cube,  3: dual GFP + RFP cube,  4: DAPI cube,  5: empty,  6: DIC analyzer
+
|detection=Spinning disc scan head Yokogawa CSU-X1: dichromatic mirrors: [http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 T-405/488/568/647]<br> [http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/561-13x15x0.5 T-405/488/561] <br>physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um<br>camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes
 +
|reflectors=Optical Insight DualView image splitter (currently not installed): <br>holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70<br>holder for GFP/RFP: HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40,  1: open,  2: 525/30,  3: razor edge 568LP,  4: dual emitter: 512/23+630/91,  5: razor edge 488LP,  6: old CSU dual emitter:  525/30 + 650/?,  7: 605/70,  8: 445/40,  9: 716/40<br> microscope filter turret:<br> position 1: GFP cube,  2: RFP cube,  3: dual GFP + RFP cube,  4: DAPI cube,  5: empty,  6: DIC analyzer
 
|features=-
 
|features=-
−
|software=iQ 1.9.1
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|software=iQ 2.5.1
 
|incubation=Stage incubator (T) + objective heater
 
|incubation=Stage incubator (T) + objective heater
 
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD1_-_Olympus_Andor_Spinning_Disc_-_startup_%26_shutdown
 
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD1_-_Olympus_Andor_Spinning_Disc_-_startup_%26_shutdown

Revision as of 16:13, 5 April 2012

Suitable for confocal imaging of EGFP, mCherry, dTomato, Draq5 and the like with high temporal and spatial resolution..
MPI19.jpg


Directions

[[image:|150px|text-bottom|right]]

MPI-CBG LMF.jpgMPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage
objectives
Olympus UPlanSApo 10x 0.4
Olympus UPlanApo 20x 0.7
Olympus UPlan FluarN 60x 0.9
Olympus UPlanSApo 60x 1.20 W
Olympus UPlanSApo 100x 1.4 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection [[detection::Spinning disc scan head Yokogawa CSU-X1: dichromatic mirrors: T-405/488/568/647
T-405/488/561
physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)
Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um
camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes]]
reflectors Optical Insight DualView image splitter (currently not installed):
holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70
holder for GFP/RFP: HC 520/35; BS 565; HC 628/40
Sutter filter wheel:
position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: 716/40
microscope filter turret:
position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer
features -
software iQ 2.5.1
incubation Stage incubator (T) + objective heater
links
  • [- beam path]
  • [ advanced system check]
inv.nr. 0 10.1.65.208


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