SD1 - Andor Spinning Disc

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(change of iQ version to the updated 2.6.1)
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|reflectors=Optical Insight DualView image splitter (currently not installed): <br>holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70<br>holder for GFP/RFP: HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40,  1: open,  2: 525/30,  3: razor edge 568LP,  4: dual emitter: 512/23+630/91,  5: razor edge 488LP,  6: old CSU dual emitter:  525/30 + 650/?,  7: 605/70,  8: 445/40,  9: edge basic 635LP<br> microscope filter turret:<br> position 1: GFP cube,  2: RFP cube,  3: dual GFP + RFP cube,  4: cube with z375 RDC for micropoint cutter,  5: empty,  6: DIC analyzer
 
|reflectors=Optical Insight DualView image splitter (currently not installed): <br>holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70<br>holder for GFP/RFP: HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40,  1: open,  2: 525/30,  3: razor edge 568LP,  4: dual emitter: 512/23+630/91,  5: razor edge 488LP,  6: old CSU dual emitter:  525/30 + 650/?,  7: 605/70,  8: 445/40,  9: edge basic 635LP<br> microscope filter turret:<br> position 1: GFP cube,  2: RFP cube,  3: dual GFP + RFP cube,  4: cube with z375 RDC for micropoint cutter,  5: empty,  6: DIC analyzer
 
|features=micro point with 15Hz 365nm cutter laser
 
|features=micro point with 15Hz 365nm cutter laser
−
|software=iQ 2.5.1
+
|software=iQ 2.6.1
 
|incubation=Stage incubator (T) + objective heater
 
|incubation=Stage incubator (T) + objective heater
 
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD1_-_Olympus_Andor_Spinning_Disc_-_startup_%26_shutdown
 
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD1_-_Olympus_Andor_Spinning_Disc_-_startup_%26_shutdown

Revision as of 16:48, 10 September 2012

Suitable for confocal imaging of EGFP, mCherry, dTomato, Draq5 and the like with high temporal and spatial resolution..
SD1 upgrade.jpg


Directions

[[image:|150px|text-bottom|right]]

MPI-CBG LMF.jpgMPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage
objectives
Olympus UPlanSApo 10x 0.4
Olympus UPlanFluar 20x 0.5
Olympus UPlan FluarN 60x 0.9
Olympus UPlanSApo 60x 1.20 W
Olympus UPlanSApo 100x 1.4 Oil
Olympus UPlanSApo 40X 0.9
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection [[detection::Spinning disc scan head Yokogawa CSU-X1 (10.000rpm):
dichromatic mirrors:
Quad band T-405/488/568/647
Triple band T-405/488/561
physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)
Andor Dual Camera Port TuCam with two cameras and 0.95x projecting lens for each camera:

Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um
Andor Neo sCMOS; dexel size of CMOS chip: 6.5um
image pixel sizes (measured April 3rd 2012):
for use with iXon: with 10x air objective: 1.695um/px (1x optovar); 1.055 um/px (1.6x opt.); with 40x air obj.: 0.418um/px (1x opt.), 0.26um/px (1.6x opt.); with 60x W obj.: 0.279um/px (1x opt.), 0.174um/px (1.6x opt.); with 100x oil obj.: 0.168um/px (1x opt.), 0.105um/px (1.6x opt.)
for use with Neo: with 10x air objective: 0.692um/px (1x optovar); 0.432um/px (1.6x opt.); with 40x air obj.: 0.17um/px (1x opt.), 0.106um/px (1.6x opt.); with 60x W obj.: 0.114um/px (1x opt.), 0.071um/px (1.6x opt.); with 100x oil obj.: 0.068um/px (1x opt.), 0.043um/px (1.6x opt.)

old measure pixel sizes before major upgrade 26th - 30th March 2012:
image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um
camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes]]

reflectors Optical Insight DualView image splitter (currently not installed):
holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70
holder for GFP/RFP: HC 520/35; BS 565; HC 628/40
Sutter filter wheel:
position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP
microscope filter turret:
position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer
features micro point with 15Hz 365nm cutter laser
software iQ 2.6.1
incubation Stage incubator (T) + objective heater
links
  • [- beam path]
  • [ advanced system check]
inv.nr. 0 10.1.65.208


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