SD1 - Andor Spinning Disc
(updated software version plus added link to iQ3 user guide) |
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|obj6=Olympus UPlanSApo 40X 0.9 | |obj6=Olympus UPlanSApo 40X 0.9 | ||
|obj7=Olympus UApo 40x 1.15 W | |obj7=Olympus UApo 40x 1.15 W | ||
+ | |obj8=Olympus UPLSAPO 40x 1.25 SIL | ||
+ | |obj9=Olympus UPLSAPO 60x 1.3 SIL | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (HBO) | |ill2=Fluorescence (HBO) | ||
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<br>old measure pixel sizes before major upgrade 26th - 30th March 2012:<br>image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um<br>camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes | <br>old measure pixel sizes before major upgrade 26th - 30th March 2012:<br>image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um<br>camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes | ||
|reflectors=Optical Insight DualView image splitter (currently not installed): <br>holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70<br>holder for GFP/RFP: HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP<br> microscope filter turret:<br> position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer | |reflectors=Optical Insight DualView image splitter (currently not installed): <br>holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70<br>holder for GFP/RFP: HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP<br> microscope filter turret:<br> position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer | ||
− | |features=micro point with 15Hz cutter laser with 365nm and 405nm dye cells | + | |features=micro point with 15Hz cutter laser with 365nm and 405nm dye cells; |
+ | objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database! | ||
|software=iQ 2.9 + iQ 3.0 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide] | |software=iQ 2.9 + iQ 3.0 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide] | ||
|incubation=Stage incubator (T) + objective heater | |incubation=Stage incubator (T) + objective heater |
Revision as of 16:06, 13 May 2014
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage | ||
objectives | |||
illumination | |||
detection | [[detection::Spinning disc scan head Yokogawa CSU-X1 (10.000rpm): dichromatic mirrors: Quad band T-405/488/568/647 Triple band T-405/488/561 Single band T-488 physical pinhole radius: 25um, physical pinhole spacing: 250um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor Dual Camera Port TuCam with two cameras and 0.95x projecting lens for each camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um | ||
reflectors | Optical Insight DualView image splitter (currently not installed): holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70 holder for GFP/RFP: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer | ||
features | micro point with 15Hz cutter laser with 365nm and 405nm dye cells;
objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!"micro point with 15Hz cutter laser with 365nm and 405nm dye cells; objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!" cannot be used as a page name in this wiki. | ||
software | [[software::iQ 2.9 + iQ 3.0 (both with Python) iQ3 user guide]] | ||
incubation | Stage incubator (T) + objective heater | ||
links |
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inv.nr. | 0 10.1.65.208 |