SD1 - Andor Spinning Disc
From BioDIP
(Difference between revisions)
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| objectives = 10x / 0.30 Plan-Neofluar<br>60x / 1.20 W UPlanSApo<br>100x / 1.40 Oil UPlanSApo | | objectives = 10x / 0.30 Plan-Neofluar<br>60x / 1.20 W UPlanSApo<br>100x / 1.40 Oil UPlanSApo | ||
| illumination = Halogen<br>HBO<br>488 nm solid-state laser Coherent Sapphire<br>561 nm solid-state laser Cobolt Jive | | illumination = Halogen<br>HBO<br>488 nm solid-state laser Coherent Sapphire<br>561 nm solid-state laser Cobolt Jive | ||
| − | | detection = Spinning disc scan head Yokogawa CSU10; back-projected pinhole | + | | detection = Spinning disc scan head Yokogawa CSU10: dichromatic mirror: [http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=855&CategoryID=82 T-405/488/568/647]<br> pinhole radius: 24um, pinhole spaceing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>image pixel size: with 100x objective: 0.16um; 100x obj. and 1.6x optovar: 0.1um; 60x obj.: 0.267um; 60x obj. and 1.6x opt.: 0.167um |
| reflectors = Optical Insight DualView image splitter: <br>HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: blocked, 8: blocked, 9: 716/40<br> microscope filter turret:<br> position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | | reflectors = Optical Insight DualView image splitter: <br>HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: blocked, 8: blocked, 9: 716/40<br> microscope filter turret:<br> position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | ||
| features = | | features = | ||
Revision as of 16:02, 22 January 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , inverted, manual, 1.6x optovar stand, Olympus IX71, fast piezo z-drive for objective | ||
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| illumination |
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| detection | [[detection::Spinning disc scan head Yokogawa CSU10: dichromatic mirror: T-405/488/568/647 pinhole radius: 24um, pinhole spaceing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size: with 100x objective: 0.16um; 100x obj. and 1.6x optovar: 0.1um; 60x obj.: 0.267um; 60x obj. and 1.6x opt.: 0.167um]] | ||
| reflectors | Optical Insight DualView image splitter: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: blocked, 8: blocked, 9: 716/40 microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | ||
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| incubation | stage incubator | ||
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| inv.nr. |
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