SD1 - Andor Spinning Disc
From BioDIP
(Difference between revisions)
(added prior xy scanning stage one change before, now: updated 561 laser power to correct value of 75mW) |
|||
Line 8: | Line 8: | ||
|room=038B | |room=038B | ||
|facility_image=logo-lmf.jpg | |facility_image=logo-lmf.jpg | ||
− | |description=Fast, "real-time" confocal imaging. Laser equipment: 488 nm solid-state Coherent Sapphire (75mW) + 561 nm solid-state Cobolt Jive ( | + | |description=Fast, "real-time" confocal imaging. Laser equipment: 488 nm solid-state Coherent Sapphire (75mW) + 561 nm solid-state Cobolt Jive (75mW). |
|applications=confocal imaging of EGFP and mCherry with high temporal and spatial resolution. | |applications=confocal imaging of EGFP and mCherry with high temporal and spatial resolution. | ||
|image=MPI19.jpg | |image=MPI19.jpg |
Revision as of 13:54, 30 September 2010
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo z-drive for objective, Prior ProScanIII xy scanning stage | ||
objectives | |||
illumination | |||
detection | [[detection::Spinning disc scan head Yokogawa CSU10: dichromatic mirror: T-405/488/568/647 physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um]] | ||
reflectors | Optical Insight DualView image splitter: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: 716/40 microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | ||
features | - | ||
software | iQ 1.9.1 | ||
incubation | Stage incubator (T) + objective heater | ||
links |
| ||
inv.nr. | 0 |