SD1 - Andor Spinning Disc
(change of iQ version to the updated 2.6.1) |
(added sgl 488 CSU dichromatic mirror) |
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Line 25: | Line 25: | ||
|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
|ill5=Laser DPSS 640 nm | |ill5=Laser DPSS 640 nm | ||
− | |detection=Spinning disc scan head Yokogawa CSU-X1 (10.000rpm):<br>dichromatic mirrors:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647]<br> [http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/561-13x15x0.5 Triple band T-405/488/561] <br>physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)<br>Andor Dual Camera Port TuCam with two cameras and 0.95x projecting lens for each camera:<br> | + | |detection=Spinning disc scan head Yokogawa CSU-X1 (10.000rpm): <br>dichromatic mirrors:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br> [http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/561-13x15x0.5 Triple band T-405/488/561] <br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T488-13x15x0.5 Single band T-488] <br>physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)<br>Andor Dual Camera Port TuCam with two cameras and 0.95x projecting lens for each camera:<br> |
Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um<br>Andor Neo sCMOS; dexel size of CMOS chip: 6.5um<br> | Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um<br>Andor Neo sCMOS; dexel size of CMOS chip: 6.5um<br> | ||
image pixel sizes (measured April 3rd 2012): <br>for use with iXon: with 10x air objective: 1.695um/px (1x optovar); 1.055 um/px (1.6x opt.); with 40x air obj.: 0.418um/px (1x opt.), 0.26um/px (1.6x opt.); with 60x W obj.: 0.279um/px (1x opt.), 0.174um/px (1.6x opt.); with 100x oil obj.: 0.168um/px (1x opt.), 0.105um/px (1.6x opt.)<br> | image pixel sizes (measured April 3rd 2012): <br>for use with iXon: with 10x air objective: 1.695um/px (1x optovar); 1.055 um/px (1.6x opt.); with 40x air obj.: 0.418um/px (1x opt.), 0.26um/px (1.6x opt.); with 60x W obj.: 0.279um/px (1x opt.), 0.174um/px (1.6x opt.); with 100x oil obj.: 0.168um/px (1x opt.), 0.105um/px (1.6x opt.)<br> |
Revision as of 14:25, 8 January 2013
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage | ||
objectives | |||
illumination | |||
detection | [[detection::Spinning disc scan head Yokogawa CSU-X1 (10.000rpm): dichromatic mirrors: Quad band T-405/488/568/647 Triple band T-405/488/561 Single band T-488 physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor Dual Camera Port TuCam with two cameras and 0.95x projecting lens for each camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um | ||
reflectors | Optical Insight DualView image splitter (currently not installed): holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70 holder for GFP/RFP: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer | ||
features | micro point with 15Hz 365nm cutter laser | ||
software | iQ 2.6.1 | ||
incubation | Stage incubator (T) + objective heater | ||
links |
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inv.nr. | 0 10.1.65.208 |