SD1 - Andor Spinning Disc

Suitable for confocal imaging of EGFP and mCherry with high temporal and spatial resolution..

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo z-drive for objective
objectives	Olympus UPlanSApo 10x 0.4 Olympus UPlanSApo 60x 1.20 W Olympus UPlanSApo 100x 1.4 Oil
illumination	Transmitted Light (Halogen) Fluorescence (HBO) Laser DPSS 488 nm Laser DPSS 561 nm
detection	[[detection::Spinning disc scan head Yokogawa CSU10: dichromatic mirror: T-405/488/568/647 physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um]]
reflectors	Optical Insight DualView image splitter: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: 716/40 microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer
features	-
software	iQ 1.9.1
incubation	Stage incubator (T) + objective heater
links	[ manual] laser power values [- beam path] [ advanced system check]
inv.nr.	0

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