SD1 - Andor Spinning Disc

Suitable for confocal imaging of EGFP, mCherry, dTomato, Draq5 and the like with high temporal and spatial resolution..

Directions

[[image:|150px|text-bottom|right]]

MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage
objectives	Olympus UPlanSApo 10x 0.4 Olympus UPlanFluar 20x 0.5 Olympus UPlan FluarN 60x 0.9 Olympus UPlanSApo 60x 1.20 W Olympus UPlanSApo 100x 1.4 Oil Olympus UPlanSApo 40X 0.9
illumination	Transmitted Light (Halogen) Fluorescence (HBO) Laser DPSS 488 nm Laser DPSS 561 nm Laser DPSS 640 nm
detection	[[detection::Spinning disc scan head Yokogawa CSU-X1 (10.000rpm): dichromatic mirrors: Quad band T-405/488/568/647 Triple band T-405/488/561 Single band T-488 physical pinhole radius: 25um, physical pinhole spacing: 250um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor Dual Camera Port TuCam with two cameras and 0.95x projecting lens for each camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um Andor Neo sCMOS; dexel size of CMOS chip: 6.5um image pixel sizes (measured April 3rd 2012): for use with iXon: with 10x air objective: 1.695um/px (1x optovar); 1.055 um/px (1.6x opt.); with 40x air obj.: 0.418um/px (1x opt.), 0.26um/px (1.6x opt.); with 60x W obj.: 0.279um/px (1x opt.), 0.174um/px (1.6x opt.); with 100x oil obj.: 0.168um/px (1x opt.), 0.105um/px (1.6x opt.) for use with Neo: with 10x air objective: 0.692um/px (1x optovar); 0.432um/px (1.6x opt.); with 40x air obj.: 0.17um/px (1x opt.), 0.106um/px (1.6x opt.); with 60x W obj.: 0.114um/px (1x opt.), 0.071um/px (1.6x opt.); with 100x oil obj.: 0.068um/px (1x opt.), 0.043um/px (1.6x opt.) old measure pixel sizes before major upgrade 26th - 30th March 2012: image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes]]
reflectors	Optical Insight DualView image splitter (currently not installed): holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70 holder for GFP/RFP: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer
features	micro point with 15Hz cutter laser with 365nm and 405nm dye cells
software	iQ 2.8
incubation	Stage incubator (T) + objective heater
links	manual laser power values [- beam path] [ advanced system check]
inv.nr.	0 10.1.65.208

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