SD2 - Andor Spinning Disc FRAPPA
From BioDIP
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=MPI12 |
| − | | system-name | + | |system-name=Andor Spinning Disc FRAPPA |
| − | | category | + | |type=Microscope |
| − | + | |category=Real-Time Confocal | |
| − | + | |facility=MPI-CBG LMF | |
| − | | facility | + | |building=MPI-CBG |
| − | + | |room=038B | |
| − | | building | + | |facility_image=logo-lmf.jpg |
| − | | room | + | |description=fast, "real-time" confocal imaging |
| − | | description | + | |applications=confocal imaging of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution. |
| − | | applications | + | |image=MPI12.jpg |
| − | | microscope | + | |stand=Inverted |
| − | | objectives | + | |microscope=motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage |
| − | | illumination | + | |objectives=10x / 0.40 UPlanSApo<br>60x / 1.20 W UPlanSApo<br>60x / 1.35 Oil UPlanSApo |
| − | | detection | + | |illumination=Cairn OptoLED lite<br>HBO<br>405nm diode laser, 50mW<br>488 nm solid-state laser Coherent Sapphire, 50mW<br>561 nm solid-state laser Cobolt Jive, 50mW<br>640nm solid-state laser, 40mW |
| − | | reflectors | + | |detection=Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU<br>image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um <br> |
| − | | features | + | |reflectors=Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) |
| − | | incubation | + | |features=Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant |
| − | | link1 | + | |incubation=stage incubator (T) + objective heater |
| − | | link2 | + | |link1=[http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsbCKRwsAwzYA LPM] |
| − | | | + | |link2=- |
| + | |obj1=- | ||
| + | |obj2=- | ||
| + | |obj3=- | ||
| + | |obj4=- | ||
| + | |obj5=- | ||
| + | |obj6=- | ||
| + | |obj7=- | ||
| + | |obj8=- | ||
}} | }} | ||
Revision as of 17:35, 1 September 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , Inverted stand, motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
| objectives | |||
| illumination |
| ||
| detection | Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um | ||
| reflectors | Optical Insight DualView image splitter: HC 520/35; BS 595 dcxr; HC 628/40 Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
| features | Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki. | ||
| software | |||
| incubation | stage incubator (T) + objective heater | ||
| links |
| ||
| inv.nr. |
