SD2 - Andor Spinning Disc FRAPPA
From BioDIP
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|room=038B | |room=038B | ||
|facility_image=logo-lmf.jpg | |facility_image=logo-lmf.jpg | ||
| − | |description= | + | |description=Fast, "real-time" confocal imaging. Illumination equipment: Cairn OptoLED lite, 405nm diode laser (50mW) + 488 nm solid-state laser Coherent Sapphire (50mW) + 561 nm solid-state laser Cobolt Jive (50mW) + 640 nm solid-state laser (40mW). |
| − | |applications=confocal imaging of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution | + | |applications=confocal imaging of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution |
|image=MPI12.jpg | |image=MPI12.jpg | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage | |microscope=motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
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|obj1=- | |obj1=- | ||
|obj2=- | |obj2=- | ||
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|obj7=- | |obj7=- | ||
|obj8=- | |obj8=- | ||
| + | |ill1=Transmitted Light (LED) | ||
| + | |ill2=Fluorescence (HBO) | ||
| + | |ill3=Laser Diode 405 nm | ||
| + | |ill4=Laser DPSS 488 nm | ||
| + | |ill5=Laser DPSS 561 nm | ||
| + | |ill6=Laser DPSS 640 nm | ||
| + | |ill7=Laser Diode 405 nm (pulsed) | ||
| + | |ill8=- | ||
| + | |detection=Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU<br>image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um <br> | ||
| + | |reflectors=Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
| + | |features=Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant | ||
| + | |incubation=stage incubator (T) + objective heater | ||
| + | |link1=[http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsbCKRwsAwzYA LPM] | ||
| + | |link2=- | ||
| + | |inv=0 | ||
| + | |objectives=10x / 0.40 UPlanSApo<br>60x / 1.20 W UPlanSApo<br>60x / 1.35 Oil UPlanSApo | ||
| + | |illumination=Cairn OptoLED lite<br>HBO<br>405nm diode laser, 50mW<br>488 nm solid-state laser Coherent Sapphire, 50mW<br>561 nm solid-state laser Cobolt Jive, 50mW<br>640nm solid-state laser, 40mW | ||
}} | }} | ||
Revision as of 16:00, 7 September 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , Inverted stand, motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
| objectives | |||
| illumination | |||
| detection | Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um | ||
| reflectors | Optical Insight DualView image splitter: HC 520/35; BS 595 dcxr; HC 628/40 Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
| features | Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki. | ||
| software | |||
| incubation | stage incubator (T) + objective heater | ||
| links |
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| inv.nr. | 0 |
