SD2 - Andor Spinning Disc FRAPPA
From BioDIP
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|stand=Inverted | |stand=Inverted | ||
|microscope=motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage | |microscope=motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
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|ill1=Transmitted Light (LED) | |ill1=Transmitted Light (LED) | ||
|ill2=Fluorescence (HBO) | |ill2=Fluorescence (HBO) | ||
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|ill6=Laser DPSS 640 nm | |ill6=Laser DPSS 640 nm | ||
|ill7=Laser Diode 405 nm (pulsed) | |ill7=Laser Diode 405 nm (pulsed) | ||
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|detection=Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU<br>image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um <br> | |detection=Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU<br>image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um <br> | ||
|reflectors=Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | |reflectors=Optical Insight DualView image splitter: <br>HC 520/35; BS 595 dcxr; HC 628/40<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) |
Revision as of 17:38, 16 September 2009
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | , Inverted stand, motorized Olympus IX81 with ZDC (hardware autofocus), 2x OptoVar, Prior NanoScanZ, Prior ProScanII xy scanning stage | ||
objectives |
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illumination | |||
detection | Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: ??um, back-projected pinhole spacing: ??um (with 100x objective, 2x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.7x before and 1.2x after the CSU image pixel size (calculated): with 60x objective: 0.131um; 60x obj. and 2.0x optovar: 0.065um; 40x obj.: 0.196um; 40x obj. and 2.0x opt.: 0.098um | ||
reflectors | Optical Insight DualView image splitter: HC 520/35; BS 595 dcxr; HC 628/40 Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: blocked, 5: blocked, 6: open, 7: polarizer for DIC, 8: 525/30, 9: blocked microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
features | Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki. | ||
software | |||
incubation | stage incubator (T) + objective heater | ||
links |
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inv.nr. | 0 |