SD2 - Andor Spinning Disc FRAPPA
From BioDIP
(Difference between revisions)
(changes done during last Andor service visit: new iQ version, different projecting lenses, diff. size of back-projected pinhole, diff. power of lasers) |
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|room=038B | |room=038B | ||
|facility_image=logo-lmf.jpg | |facility_image=logo-lmf.jpg | ||
| − | |description=Fast, "real-time" confocal imaging. Illumination equipment: Cairn OptoLED lite, 405nm diode laser ( | + | |description=Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: Cairn OptoLED lite, 405nm diode laser (100mW) + 488 nm solid-state laser Coherent Sapphire (50mW) + 561 nm solid-state laser Cobolt Jive (50mW) + 640 nm solid-state laser (100mW). Optional: 405 pulsed laser for cutting. |
| − | |applications=confocal imaging of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution | + | |applications=confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution |
|image=MPI12.jpg | |image=MPI12.jpg | ||
|stand-name=Olympus - IX81 | |stand-name=Olympus - IX81 | ||
|stand=Inverted | |stand=Inverted | ||
| − | |microscope=motorized with ZDC (hardware autofocus), 2x | + | |microscope=motorized with ZDC (hardware autofocus), 2x Optovar, Prior NanoScanZ, Prior ProScanIII xy scanning stage |
|obj1=Olympus UPlanSApo 10x 0.4 | |obj1=Olympus UPlanSApo 10x 0.4 | ||
|obj2=Olympus UPlanSApo 60x 1.20 W | |obj2=Olympus UPlanSApo 60x 1.20 W | ||
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|ill6=Laser DPSS 640 nm | |ill6=Laser DPSS 640 nm | ||
|ill7=Laser Diode 405 nm (pulsed) | |ill7=Laser Diode 405 nm (pulsed) | ||
| − | |detection=Spinning disc scan head Yokogawa CSU-X1; back-projected pinhole size: 0. | + | |detection=Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>note there are two additional lenses in the optical path: 1.0x before and 1.9x after the CSU<br>image pixel size (calculated): with 60x objective: 0.14um; 60x obj. and 2.0x optovar: 0.07um; 40x obj.: 0.21um; 40x obj. and 2.0x opt.: 0.11um <br> |
|reflectors=Optical Insight DualView image splitter: <br> insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting<br>insert with T565 lpxr DC and empty EM position<br>insert for CFP/YFP splitting<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | |reflectors=Optical Insight DualView image splitter: <br> insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting<br>insert with T565 lpxr DC and empty EM position<br>insert for CFP/YFP splitting<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
|features=Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant | |features=Andor FRAPPA<br>405nm pulsed diode laser from PicoQuant | ||
| − | |software=iQ 1. | + | |software=iQ 1.10.2 [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:IQ_1_10_2_User_Guide.pdf user guide] |
|incubation=Stage incubator (T) + objective heater | |incubation=Stage incubator (T) + objective heater | ||
|link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsbCKRwsAwzYA | |link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsbCKRwsAwzYA | ||
Revision as of 11:43, 14 May 2010
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Olympus - IX81, Inverted stand, motorized with ZDC (hardware autofocus), 2x Optovar, Prior NanoScanZ, Prior ProScanIII xy scanning stage | ||
| objectives | |||
| illumination | |||
| detection | Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um note there are two additional lenses in the optical path: 1.0x before and 1.9x after the CSU image pixel size (calculated): with 60x objective: 0.14um; 60x obj. and 2.0x optovar: 0.07um; 40x obj.: 0.21um; 40x obj. and 2.0x opt.: 0.11um | ||
| reflectors | Optical Insight DualView image splitter: insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting insert with T565 lpxr DC and empty EM position insert for CFP/YFP splitting Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36 microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
| features | Andor FRAPPA 405nm pulsed diode laser from PicoQuant"Andor FRAPPA<br />405nm pulsed diode laser from PicoQuant" cannot be used as a page name in this wiki. | ||
| software | [[software::iQ 1.10.2 user guide]] | ||
| incubation | Stage incubator (T) + objective heater | ||
| links |
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| inv.nr. | 0 |
