SD2 - Andor Spinning Disc FRAPPA
From BioDIP
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|building=MPI-CBG | |building=MPI-CBG | ||
|room=038B | |room=038B | ||
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|description=Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: Cairn OptoLED lite, 405nm diode laser (100mW) + 488 nm solid-state laser Coherent Sapphire (50mW) + 561 nm solid-state laser Cobolt Jive (50mW) + 640 nm solid-state laser (100mW). Optional: 405 pulsed laser for cutting. | |description=Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: Cairn OptoLED lite, 405nm diode laser (100mW) + 488 nm solid-state laser Coherent Sapphire (50mW) + 561 nm solid-state laser Cobolt Jive (50mW) + 640 nm solid-state laser (100mW). Optional: 405 pulsed laser for cutting. | ||
|applications=confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution | |applications=confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution | ||
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|detection=Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>Andor Dual camera port with two cameras: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>Andor Clara cooled interline CCD; pixel size of chip: 6.45um<br>note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.067x (was 1.9x before addition of dual camera switch) in front of iXon or a 1.2x in front of Clara<br>image pixel size for use with iXon (calculated): with 100x: 0.077um; with 60x objective: 0.129 (was 0.14um); 60x obj. and 2.0x optovar: 0.0645um (was 0.07um); 40x obj.: 0.194um (was 0.21um); 40x obj. and 2.0x opt.: 0.0967um (was 0.11um) <br> | |detection=Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>Andor Dual camera port with two cameras: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>Andor Clara cooled interline CCD; pixel size of chip: 6.45um<br>note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.067x (was 1.9x before addition of dual camera switch) in front of iXon or a 1.2x in front of Clara<br>image pixel size for use with iXon (calculated): with 100x: 0.077um; with 60x objective: 0.129 (was 0.14um); 60x obj. and 2.0x optovar: 0.0645um (was 0.07um); 40x obj.: 0.194um (was 0.21um); 40x obj. and 2.0x opt.: 0.0967um (was 0.11um) <br> | ||
|reflectors=Optical Insight DualView image splitter: <br> insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting<br>insert with T565 lpxr DC and empty EM position<br>insert for CFP/YFP splitting<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | |reflectors=Optical Insight DualView image splitter: <br> insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting<br>insert with T565 lpxr DC and empty EM position<br>insert for CFP/YFP splitting<br>Sutter filter wheel: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36<br> microscope filter turret:<br> position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
− | |features= | + | |features=- |
|software=iQ 1.10.3 [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:IQ_1_10_2_User_Guide.pdf user guide] + iQ 2.0 (with Python) | |software=iQ 1.10.3 [https://ifn.mpi-cbg.de/wiki/ifn/index.php/File:IQ_1_10_2_User_Guide.pdf user guide] + iQ 2.0 (with Python) | ||
|incubation=Stage incubator (T) + objective heater | |incubation=Stage incubator (T) + objective heater | ||
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|link2=- | |link2=- | ||
|inv=0 | |inv=0 | ||
+ | |facility_image=logo-lmf.jpg | ||
|objectives=10x / 0.40 UPlanSApo<br>60x / 1.20 W UPlanSApo<br>60x / 1.35 Oil UPlanSApo | |objectives=10x / 0.40 UPlanSApo<br>60x / 1.20 W UPlanSApo<br>60x / 1.35 Oil UPlanSApo | ||
|illumination=Cairn OptoLED lite<br>HBO<br>405nm diode laser, 50mW<br>488 nm solid-state laser Coherent Sapphire, 50mW<br>561 nm solid-state laser Cobolt Jive, 50mW<br>640nm solid-state laser, 40mW | |illumination=Cairn OptoLED lite<br>HBO<br>405nm diode laser, 50mW<br>488 nm solid-state laser Coherent Sapphire, 50mW<br>561 nm solid-state laser Cobolt Jive, 50mW<br>640nm solid-state laser, 40mW | ||
}} | }} |
Revision as of 17:38, 31 May 2011
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX81, Inverted stand, motorized with ZDC (hardware autofocus), 2x Optovar, Prior NanoScanZ, Prior ProScanIII xy scanning stage | ||
objectives | |||
illumination | |||
detection | Spinning disc scan head Yokogawa CSU-X1; pinhole size: 24um, pinhole spacing: 240um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) Andor Dual camera port with two cameras: Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um Andor Clara cooled interline CCD; pixel size of chip: 6.45um note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 2.067x (was 1.9x before addition of dual camera switch) in front of iXon or a 1.2x in front of Clara image pixel size for use with iXon (calculated): with 100x: 0.077um; with 60x objective: 0.129 (was 0.14um); 60x obj. and 2.0x optovar: 0.0645um (was 0.07um); 40x obj.: 0.194um (was 0.21um); 40x obj. and 2.0x opt.: 0.0967um (was 0.11um) | ||
reflectors | Optical Insight DualView image splitter: insert with HC 520/35; BS 595 dcxr; HC 628/40 for GFP/RFP splitting insert with T565 lpxr DC and empty EM position insert for CFP/YFP splitting Sutter filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36 microscope filter turret: position 1: empty, 2: DIC, 3: tripple DAPI + GFP + RFP cube, 4: GFP, 5: RFP, 6: DAPI cube (X 345/10; D 380LP; M 445/40) | ||
features | - | ||
software | [[software::iQ 1.10.3 user guide + iQ 2.0 (with Python)]] | ||
incubation | Stage incubator (T) + objective heater | ||
links |
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inv.nr. | 0 |